近红外光谱快速检测食用油必需脂肪酸  

Rapid determination of essential fatty acids in edible oils based on near infrared spectroscopy

作　　者：张辉[1] 吴迪[1] 李想[1] 石品艳[1] 王思寒[1] 冯凤琴[1] 何勇[1] 

机构地区：[1]浙江大学生物系统工程与食品科学学院,杭州310029

出　　处：《农业工程学报》2012年第7期266-270,共5页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家科技支撑计划(No.2006BAD05A02);国家高技术研究发展计划("863"计划)项目(2007AA10Z210)

摘　　要：为了建立食用油必需脂肪酸快速检测的方法,该研究提出了基于近红外光谱技术检测食用油中α-亚麻酸和亚油酸含量的快速测定方法。对光谱信息分别采用偏最小二乘回归方法(PLS)和最小二乘支持向量机(LS-SVM)建立模型。比较了多种光谱预处理方法对模型预测能力的影响。结果表明对于亚油酸含量的预测,采用Savitzky-Golay平滑法结合多元散射校正(MSC)的光谱预处理所建立的LS-SVM模型最优。预测集的决定系数(R2)、预测均方根误差(RMSEP)和剩余预测偏差(RPD)分别达到了0.989,0.0161和9.4783。对于α-亚麻酸含量的预测,采用Savitzky-Golay平滑法结合标准正态变换(SNV)的光谱预处理所建立的LS-SVM模型最优。α-亚麻酸含量预测结果的R2、RMSEP和RPD为0.972,0.0036和6.0561,据此表明,应用近红外光谱技术能够检测食用油中α-亚麻酸和亚油酸的含量,为快速检测食用油的必需脂肪酸提供了参考。In order to find out a fast quantitative determination method for the essential fatty acids in edible oils,near infrared spectroscopy was applied in determination of the contents ofα-linolenic acid and linoleic acid.The chemometrics models between near infrared spectra and the contents ofα-linolenic acid and linoleic acid were established by partial least squares regression（PLS）and least-squares support vector machine（LS-SVM）.Several common used spectral pretreatment methods were used to establish different PLS and LS-SVM models.For linoleic acid prediction,best predictive performance was obtained using LS-SVM model and spectral pretreatment of Savitzky-Golay smoothing and multiplicative scatter correction（MSC）.Coefficient of determination（Rp 2）,root mean square error for prediction（RMSEP）and residual predictive deviation（RPD）were 0.0161,0.989 and 9.4783,respectively.For α-linolenic acid prediction,best predictive performance was obtained using LS-SVM model and the spectral pretreatment of Savitzky-Golay smoothing and standard normal variation（SNV）.Rp 2,RMSEP and RPD were 0.0036,0.972 and 6.0561,respectively.The results indicate that it is feasible to use near infrared spectroscopy for fast determination ofα-linolenic acid and linoleic acid contents in edible oils.

关 键 词：近红外光谱 模型 优化 α-亚麻酸和亚油酸 食用油 检测 

分 类 号：O657.33[理学—分析化学] TS225.1[理学—化学]