RP-HPLC法测定石榴汁中鞣花酸的含量  

Determination of ellagic acid in the pomegranate juice by RP-HPLC

作　　者：彭海燕[1] 陈祥贵[1] 刘振平[1] 何宇新 杨文宇[1] 杨潇[1] 李玉锋[1] 

机构地区：[1]西华大学生物工程学院食品生物技术四川省高校重点实验室,成都610039

出　　处：《食品科技》2012年第4期283-285,共3页Food Science and Technology

基　　金：国家星火计划项目(2010GA810002);四川省科技攻关项目(05SG011-021)

摘　　要：目的:建立检测石榴汁中鞣花酸的含量测定方法。方法;采用高效液相色谱法,ArcusEP-C18(250mm×4.6mm,5μm)色谱柱,以甲醇-0.1%TFA为流动相梯度洗脱,流速1.0mL/min,检测波长254nm。结果:鞣花酸0.004～0.064μg之间线性关系良好,平均加样回收率为99.32%。结论:方法精密可靠,可作为检测石榴汁中鞣花酸含量的方法。Objective：To establish HPLC method for determination of ellagic acid in the pomegranate juice.Method：The RP-HPLC was carried out on Arcus EP-C 18（250 mm×4.6 mm,5 μm）.The gradient elution was performed by a mobile phase consisted a mixture of methanol and 0.1% TFA at a flow rate of 1.0 mL/min,and detection wavelength was at 254 nm.Results：The linear range was 0.004～0.064 μg for ellagic acid.The average recoveries was 99.32%.Conclusion：The method is accurate and reproducible for quality determination of ellagic acid in pomegranate juice.

关 键 词：石榴汁 鞣花酸 反相高效液相色谱法 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]