葡萄基因组密码子使用偏好模式研究  

Codon Usage Bias in Vitis vinifera

作　　者：续晨[1] 蔡小宁[1] 钱保俐[1] 贲爱玲[1] 

机构地区：[1]南京晓庄学院生物化工与环境工程学院,南京211171

出　　处：《西北植物学报》2012年第2期409-415,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：江苏省高校自然科学基金(08KDJ180011);南京晓庄学院青年专项基金项目(NXZ09039)

摘　　要：根据完整基因组序列,运用多元统计分析和对应分析的方法,探讨了葡萄全基因组序列密码子的使用模式和影响密码子使用的各种可能因素。结果显示:葡萄密码子偏好性主要受到碱基差异(r=0.925)和自然选择(r=0.193)共同作用的影响,突变压力占了主导因素,自然选择的作用较小。同时基因长度和蛋白质疏水性也对密码子的偏好性有所影响。确定了葡萄的20个最优密码子。In this study, trends in synonymous codon usage in Vitis vinifera have been firstly examined through the multivariate statistical analysis on 1374 cDNA sequences. The results showed that the genes positions on the primary axis were strongly negatively correlated with GC3s,GC content of individual gene and gene expression level, which indicated that nucleotide composition and gene expression level were the main factors in shaping the codon usage of V. vinifera,and the variation in codon usage among genes may be due to mutational bias （r=0. 925） at the DNA level and natural selection （r=0. 193） acting at the level of mRNA translation. At the same time,CDS length and the hydrophobicity of each protein were,respectively,significantly correlated with codon bias.

关 键 词：分子进化 密码子用法 密码子偏好性 

分 类 号：Q786[生物学—分子生物学]