基于响应面法优化玉米秸秆酶解条件  

Optimization of enzyme hydrolysis conditions of corn stalk by response surface methodology

作　　者：任天宝[1,2] 常娟 康玉鹤 任秀娟[1] 宋安东 张百良 

机构地区：[1]河南科技学院资源与环境学院,河南新乡453003 [2]农业部农村可再生能源重点实验室,河南郑州450002 [3]鹤壁市互邦农业科技有限公司,河南鹤壁458003

出　　处：《河南农业大学学报》2012年第1期76-80,共5页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(70741032);国家农业科技成果转化资金项目(2006GB2D000173)

摘　　要：以蒸汽爆破玉米秸秆为原料,基于Box-Behnken试验设计,选取MgSO4、吐温80(Tween80)和牛血清白蛋白(BSA)作为酶解体系添加物,采用响应面法优化了玉米秸秆的酶解条件,并建立了数学模型.结果表明,在玉米秸秆酶解体系中,分别添加质量浓度为0.62 mg.g-1 MgSO4(底物),53.0 mg.g-1 Tween80(底物),0.48 mg.g-1 BSA(底物),48 h糖化后,还原糖质量浓度从60.05 g.L-1提高到73.64 g.L-1,比对照提高了22.63%,糖化率质量分数达到理论值的85.38%,并验证了数学模型的有效性,获得较高的还原糖质量浓度.表明添加适量的化学物质,可以显著提高还原糖质量浓度.Abstract： Based on Box-Behnken design, MgSQ, Tween80 and Bovine serum albumin （BSA）were chosen as additives for enzyme hydrolysis. The process conditions of corn stalk by steam explosion pre- treatment were optimized by response surface methodology, and a mathematical model of a second order quadratic equation was developed for reducing sugar concentration. The results showed that the optimized technological parameters were as follows：the optimal addition of these three chemical inducers for MgSO4, Tween80 and BSA were 0.62, 53.0 and 0.48 mg ·g-1, respectively. After saccharification under these conditions for 48 h, the final concentration of reducing sugar was increased to 73.64 g ·L-1 from 60.05 g·L-1 of the control, and the saccharification rate reached 85.38% of its theoreti- cal value. The results verified the validity of the mathematical model. This study demonstrates that the addition of proper amount chemical inducers can significantly improve the concentration of reducing sugar.

关 键 词：玉米秸秆 蒸汽爆破 化学物质 酶解 响应面法 

分 类 号：S216.2[农业科学—农业机械化工程]