电极干预对青霉素致痫大鼠痫灶电势及ATP合成酶β亚基表达变化的影响  

Effects of Electrode on Epileptogenic Focus Potential and Expressions of the β Subunit of ATP Synthase in Rats with Penicillin-induced Epilepsy

作　　者：贺国华[1,2] 郑文[1] 康庆云[1] 田婧[1] 黄献[1] 宋治[1] 

机构地区：[1]中南大学湘雅三医院神经内科,长沙410013 [2]衡阳市中心医院神经内科

出　　处：《生物医学工程学杂志》2012年第2期287-290,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30971534)

摘　　要：为探讨青霉素致痫大鼠脑内生物能量的变化过程及电极干预对其的影响,将50只SD大鼠随机分为生理盐水对照组(A组)、青霉素模型组(B组)、金属电极干预组(C组)及绝缘电极干预组(D组),测定致痫灶电势及海马ATP合成酶β亚基(ATP5B)表达。发现致痫灶内电势及海马神经元细胞ATP5B表达呈现先增强后减弱变化趋势,金属电极置入降低了致痫灶电势而不改变海马ATP5B表达。推测青霉素致痫大鼠痫性发作过程中存在脑内电势的变化,出现相应的生物能量代谢的变化,致痫灶电势因金属电极干预而消减。The changed process of bioenergy and the effects of electrode interfering on penicillin-induced epileptic brains in epileptic seizures rats were investigated.Fifty Sprague-Dawley（SD） rats were randomly divided into 4 groups,i.e.normal saline control group（group A）,penicillin model group（group B）,metal electrode interfere group（group C） and insulated electrode interfere group（group D）.The epileptogenic potential and the expressions of the β subunit of-ATP synthase（ATP5B） in hippocampal neurons were measured.The epileptogenic foucus potential and expressions of ATP5B in hippocampus neurons showed that the trend increased at first and decreased implantation of later,and the implantation of metal electrodes decreased the epileptogenic foucus potential at corresponding time point,but had no effect on the expressions of ATP5B.The change of epileptogenic focus potential was reduced by implantation of metal electrode,possibly due to the alteration of corrosponding bioenergy metabolism which had participated in the process of epileptic seizure.

关 键 词：癫痫 青霉素 ATP合成酶β亚基 生物能量 

分 类 号：R742.1[医药卫生—神经病学与精神病学]