转化生长因子-β1在大鼠腹膜间皮细胞转分化中的作用及机制  

Effects of TGF-β 1 on epithelial-mesenchymal transition of rat peritoneal mesothelial cells

作　　者：刘小贤[1] 周红娟[1] 汪卫[1] 于健宁[1] 马纪林[1] 

机构地区：[1]浙江省中西医结合医院肾内科,杭州310003

出　　处：《浙江医学》2012年第6期408-411,共4页Zhejiang Medical Journal

基　　金：浙江省自然科学基金项目（Y2101241）

摘　　要：目的 研究转化生长因子-β1（TGF-β1）在大鼠腹膜间皮细胞（RPMCs）转分化中的作用及机制.方法 体外培养SD大鼠原代腹膜间皮细胞,静止24h后,随机分为正常对照组（不加TGF-β1处理）及TGF-β1（10ng/ml）刺激组.采用RT-PCR法及Western blotting检测TGF-β1处理后不同时点NADPH氧化酶亚单位p67phox、α-平滑肌肌动蛋白（α-SMA）、E-钙粘素（E-cadherin）及Ⅰ型胶原（CollagenⅠ）mRNA和蛋白的表达情况.结果 TGF-β1刺激RPMCs能导致E-cadherin mRNA和蛋白表达下调,p67phox、α-SMA、CollagenⅠmRNA和蛋白表达上调,呈现一定的时间依赖性.结论 TGF-β1诱导了大鼠腹膜间皮细胞转分化,NADPH氧化酶可能在其转分化过程中发挥重要作用.Objective To investigate the effects of TGF-β 1 on EpitheliaI-Mesenchymal Transition of rat peritoneal mesothelial cells （RPMCs） Methods After synchronization for 24 h, primarily cultured rat RPMCs were randomly assigned to 2 groups： group A （control）, group B （TGF- β, 1, 10ng/ml）. Growth arrested and synchronized RPMCs were stimulated by 10ng/ml TGF- β 1 for different times. The mRNA and protein expression levels of NADPH oxidase subunit p67phox, E-cadherin, α -SMA and Collagen I were measured by RT-PCR and Western blotting, respectively. Results TGF- β 1 up-regulated mRNA and protein expression of NADPH oxidase subunit p67phox, α-SMA and Collagen I , and down-regulate mRNA and protein expression of E-cadherin in rat RPMCs. Conclusion RPMCs can be transdifferentiated into myofibroblast with TGF- β1, which may be related to the activation of NADPH oxidase.

关 键 词：腹膜间皮细胞 转分化 TGF—B NADPH 氧化酶 

分 类 号：R575.2[医药卫生—消化系统]