Green-ripe(Gr)基因植物表达载体的构建及在Micro-Tom番茄中的遗传转化  

Construction of Green-ripe(Gr) Gene Plant Expression Vector and Transgenic in Tomato

作　　者：杨鹏跃[1] 王云香[1] 李玲[1] 朱本忠[1] 傅达奇[1] 罗云波[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院食品生物技术实验室,北京100083

出　　处：《中国食品学报》2012年第3期8-12,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(No.30871741;No.30972037)

摘　　要：Green-ripe(Gr)是一种番茄果实成熟突变体,有报道表明番茄中的Gr基因以某种形式参与乙烯信号转导途径,导致番茄果实成熟过程中的乙烯不敏感特性。从番茄果实中克隆Gr基因,成功构建Gr基因的植物表达载体pCAMBIA-1300-Gr,并以Micro-Tom番茄品种为材料,采用农杆菌介导叶盘法转化得到转基因植株,经PCR初步验证为阳性植株,为进一步研究Gr基因的功能奠定了基础。Green-ripe（Gr） is one kind of tomato mutants, which could lead to the ethylene insensitivity during the maturity of the tomato fruit. It was reported that Gr gene might take part in the ethylene signal transduction pathway. In this paper, in order to perfect the function of the Gr gene, the Gr gene was successfully connected to the pCAMBIA- 1300 vector which became the plant expression vector. Agrobacterium-mediated leaf disc transformation which was used to introduce the Cr gene into tomato （Micro-TOM） was studied. The transformed plants obtained were identified by PCR analysis, which showed that the target gene was transferred into the plants. By this way, the efficient tomato transforma- tion system was developed in order to identify more about the function of the gene Gr.

关 键 词：Green-ripe Micro-Tom番茄 遗传转化 

分 类 号：S641.2[农业科学—蔬菜学]