叶黄素的分离纯化及其清除羟自由基活性  

Separation and Purification of Lutein and Its Scavenging Activities on Hydroxyl Radical

作　　者：彭胜[1] 彭密军[1] 黄美娥[1] 张昌伟[1] 张琳杰[1] 

机构地区：[1]吉首大学林产化工工程湖南省重点实验室

出　　处：《食品与发酵工业》2012年第3期145-148,共4页Food and Fermentation Industries

基　　金：湖南省教育厅重点项目(08A056);湖南省自然科学基金项目(10JJ6024);湖南省高校"林产资源化学与林化产品开发"科技创新团队支持计划项目(湘教通[2010]212号)

摘　　要：运用KOH-甲醇溶液皂化法将叶黄素酯转化为游离叶黄素,进一步采用硅胶柱层析进行分离纯化,并对不同纯度产品中叶黄素的含量进行HPLC法测定;以没食子酸丙酯为参照,采用流动注射化学发光法测定不同纯度叶黄素产品清除.OH的效果。结果表明:叶黄素浸膏通过皂化,游离叶黄素的含量由1.35%提高到30.25%,进一步柱层析处理得到80.32%的叶黄素产品;不同纯度叶黄素产品均具有较强的清除.OH活性,且其抗氧化活性与叶黄素的含量正相关。In this paper,the luteinesters was saponated and purified by KOH-methanol methods and silica gel column chromatography,and the content was determined by HPLC.Propyl gallate was used as a positive control,the products of lutein were assessed for their antioxidative effects on hydroxyl.The results demonstrated that the content of lutein was successively increased from 1.35% to 30.25% and 80.32% by saponification and purification.The products of lutein showed obvious scavenging ability to hydroxyl radical,and its antioxidant activity was positively correlated with the content.

关 键 词：叶黄素 分离纯化 羟自由基 流动注射化学发光法 

分 类 号：R151[医药卫生—营养与食品卫生学]