多粘菌素B与内毒素相互作用的红外光谱分析  

Analysis of FTIR on interaction between PMB and endotoxin

作　　者：张波[1] 何建川[1] 邵阳[2] 

机构地区：[1]川北医学院基础医学院,四川南充637000 [2]川北医学院医学影像研究所,四川南充637000

出　　处：《西部医学》2012年第4期640-642,共3页Medical Journal of West China

基　　金：四川省教育厅重点项目(NO:10ZA078)

摘　　要：目的利用傅里叶变换红外光谱(fourier transform infrared spectroscopy,FTIR)探讨多粘菌素B(polymyxcin B,PMB)与内毒素的相互作用力。方法利用FTIR分析PMB与内毒素相互作用后的红外光谱图。结果①PMB的N-H伸缩振动:在PMB与内毒素吸附后的图谱中该吸收峰的强度相比PMB的吸收峰强度有所减弱。②C-N的伸缩振动:PMB与内毒素吸附后的图谱中该吸收峰的强度相比PMB的吸收峰强度也有一定程度的减弱。结论PMB作为吸附剂吸附内毒素的作用力是正、负电荷间的静电引力。Objective To study interactionforce between PMB and endotoxin use fourier transform infrared spectroscopy（FTIR）.Methods The infrared spectrogram of polymyxcin B（PMB） absorbing endotoxin use FTIR were analyzed.Results The aborption of N-H stretching vibration of PMB absobing endotoxin decreased compared to which of PMB.The absorption of C-N stretching vibration of PMB absobing endotoxin decreased compared to which of PMB.Conclusion The electrostatic attraction between positive charge and negative charge is just the interactionforce of PMB absobing endotoxin.

关 键 词：傅里叶变换红外光谱 多粘菌素B 内毒素 作用 

分 类 号：R363.122[医药卫生—病理学]