纳米氧化锌对人正常肝细胞DNA及染色体的损伤作用  

DNA and chromosome damage of ZnO nanoparticles on human hepatocyte cells

作　　者：关荣发[1] 陈潇婷[1] 芮昶[2] 蒋家新[1] 刘明启[1] 刘光富[1] 

机构地区：[1]中国计量学院生命科学学院生物计量及检验检疫技术重点实验室,浙江杭州310018 [2]杭州质量技术监督检测院,浙江杭州310019

出　　处：《中国药理学与毒理学杂志》2012年第2期237-241,共5页Chinese Journal of Pharmacology and Toxicology

基　　金：国家高技术研究发展计划(863计划)资助项目(2007AA100403);浙江省自然科学基金(Y2110952);浙江省实验动物科技计划项目(2011C37075)~~

摘　　要：目的探讨纳米氧化锌对人正常肝细胞HL7702的细胞毒性和遗传毒性作用。方法纳米氧化锌10,25,50,75和100 mg.L-1培养HL7702细胞12,24和48 h。MTT法检测进行毒性评级(RGR)。单细胞凝胶电泳测定细胞头部DNA百分率、尾部DNA百分率、尾矩和Olive尾矩;微核试验测定微核率。结果纳米氧化锌对HL7702肝细胞的半数抑制浓度(IC50)为29.81 mg.L-1。纳米氧化锌10,25,50,75和100 mg.L-1作用细胞24 h,RGR分别为(96±3)%,(83±3)%,(52±4)%,(41±3)%和(21±2)%,毒性级别从1级升到4级。与正常对照组相比,纳米氧化锌10,25,50,75和100 mg.L-1组细胞DNA均有不同程度损伤,HL7702细胞的尾矩和Olive尾矩均呈逐渐显著升高趋势,头部DNA百分率降低,尾部DNA百分率增加。微核试验结果显示,12 h时纳米氧化锌≥50 mg.L-1微核率显著升高,24 h时,纳米氧化锌≥25 mg.L-1微核率显著升高;48 h时,纳米氧化锌10,25,50,75和100 mg.L-1微核率分别升高到8.3%,9.2%,17.3%和21.7%(P<0.05)。结论纳米氧化锌对HL7702肝细胞具有细胞毒性和遗传毒性。OBJECTIVE To explore cytotoxicity and genetoxic effect of ZnO nanoparticles on human hepatocyte cells（HL7702）,and to provide a scientific basis about evaluating the safety exposure to ZnO nanoparticles.METHODS Human hepatocyte cells were exposed to ZnO nanoparticles 10,25,50,75 and 100 mg·L-1 for 12,24 and 48 h.Relative growth rate（RGR） was detected by MTT assay.DNA damage and chromosomal damage were evaluated using head DNA percentage,tail DNA percentage,tail moment（TM）,Olive tail moment（OTM） and micronucleus rate by SCGE singlecelgeleletrophoresis.RESULTS The half inhibitory concentration（IC50） of ZnO nanoparticles for HL7702 cells was 29.81 mg·L-1.Compared with normal control group,cell activity in ZnO nanoparticles 10,25,50,75 and 100 mg·L-1 groups significantly decreased.The tail moment,Olive tail moment and tail DNA percentage significantly increased with rising of ZnO nanoparticles concentration,however head DNA percentage decreased.Micronucleus assay showed that when cells exposed to ZnO nanoparticles 50 and 100 mg·L-1 for 12 and 24 h,micronuclear rates significantly increased（P0.05）,and when cells exposed to ZnO nanoparticles 10,25,50 and 100 mg·L-1 for 48 h,micronuclear rates increased to 8.3%,9.2%,13.7% and 21.7%,respectively（P0.05）.CONCLUSION ZnO nanoparticles have the cytotoxicity and genotoxicity on HL7702 cells in vitro.

关 键 词：氧化锌 纳米粒子 肝细胞 DNA损伤 微核试验 

分 类 号：R363[医药卫生—病理学]