RalA RhoC在非小细胞肺癌组织中的表达及意义  

The Expression and Significance of RhoC and RalA in Non-Small Cell Lung Carcinoma

作　　者：陈丹[1] 于壮[1] 潘华政[1] 杨堃[1] 刘相萍[1] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003

出　　处：《现代生物医学进展》2012年第7期1296-1299,共4页Progress in Modern Biomedicine

基　　金：青岛市科技计划项目(06-2-2-5-nsh-1);山东省科技发展计划项目(2010GWZ20206)

摘　　要：目的:观察RalA和RhoC基因在非小细胞肺癌组织细胞中的表达变化,探讨它们的表达在非小细胞肺癌发展和侵袭转移中的作用。方法:通过实时荧光定量PCR(FQ-PCR)检测RalA、RhoC在60例非小细胞肺癌患者的肿瘤组织和癌旁组织细胞中的表达,了解其与非小细胞肺癌临床病理学指标的关系。结果:RhoC在非小细胞肺癌组织细胞中的表达较癌旁组织显著增高(P<0.05),且与淋巴结的转移、TNM分期和分化程度密切相关(P<0.05)。而RalA在非小细胞肺癌组织细胞中的表达量低于癌旁组织(P<0.05),与淋巴结转移无明显相关(P>0.05)。二者在鳞、腺癌中表达均无差异(P<0.05)。结论:肺癌组织细胞中RhoC表达升高及RalA表达异常下调与非小细胞肺癌的发生、发展及侵袭机制关系密切。Objective： To investigate the mRNA express of Ral A and RhoC in lung carcinoma and their clinical significance.Methods： The quantity of RhoC and RalA were detected by TaqMan probe based fluorescence quantitative polymerase chain reaction（FQ-PCR） in 60 cases of lung carcinoma,corresponding para-cancer tissues.Results： In lung carcinoma,the mean quanitities of RhoC mRNA were higher than that in corresponding para-cancer tissues（P0.05）,and was closely related with lymph node metastases;TNM stage and histological grade.The mean quanitities of RalA mRNA was higher in corresponding para-cancer tissues（p0.05）,and no significant association was found bettwen RalA and lymph node metastases（P0.05）.In lung caner,both RalA and RhoC mRNA have no difference between squamous cell carcinoma and adenocarcinoma.Conclusion： Preserved expressin of Rho C and defective expressin of RalA can be concerned with NSCLC.

关 键 词：非小细胞肺癌 RHOC RALA 荧光定量PCR 

分 类 号：R734.2[医药卫生—肿瘤]