不同工艺萃取逍遥散中挥发性成分的气相色谱-质谱联用比较分析  

Comparative Analysis of Volatile Components Extracted from Xiao-yao-san Formula with SFE-CO_2 and Steam Distillation by GC-MS

作　　者：郭晓擎[1,2] 田俊生[1] 贾广成[1,2] 周玉枝[1] 李金兵[1,2] 秦雪梅[1] 

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学化学化工学院,山西太原030006

出　　处：《时珍国医国药》2012年第4期839-841,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(No.81173366;31070295);国家自然科学基金青年基金(No.81102833)

摘　　要：目的比较分析不同工艺萃取所得传统中药复方逍遥散中挥发性成分。方法采用超临界CO2流体萃取法(SFE-CO2)和水蒸气蒸馏法萃取逍遥散中挥发性成分,并使用气相色谱-质谱联用(GC-MS)结合计算机解析技术对其化学成分进行分析鉴定。结果超临界CO2流体法所得成分分离得到40个色谱峰,可鉴定化合物有25种,水蒸气蒸馏法所得成分分离得到115个色谱峰,可鉴定化合物有50种。结论水蒸气蒸馏法更适合逍遥散挥发性成分的提取。ObjectiveTo compare and analyze the volatile constituents extracted from compound traditional Chinese medicine Xiao-yao-san formula by different processes. MethodsXiao-yao-san prescriptions were extracted by supercritical fluid extraction（SFE-CO2） and steam distillation,respectively.The volatile samples were analyzed and identified by GC-MS with computer-based retrieval. ResultsIn total,40,115 peaks were determined and 25,50 compounds in Xiao-yao-san volatile constituents extracted by SFE-CO2 and SD,respectively. ConclusionSteam distillation is more suitable for the extraction of volatile constituents in compound Xiao-yao-san formula.

关 键 词：逍遥散 超临界CO2 蒸馏 挥发性成分 气相色谱-质谱联用 

分 类 号：R284.1[医药卫生—中药学]