流式细胞术在检测细胞内活性氧中的应用  

Application of Flow Cytometry in Detection of Cellular Reactive Oxygen Species

作　　者：赵凯[1,2] 陈琦[1] 王利[1] 贾宇臣[1] 

机构地区：[1]内蒙古医学院,呼和浩特010059 [2]呼和浩特市第一医院,呼和浩特010010

出　　处：《现代检验医学杂志》2012年第1期99-100,103,共3页Journal of Modern Laboratory Medicine

基　　金：基金项目：内蒙古医学院科技百万基金项目（NY2011BW002）.

摘　　要：目的流式细胞术检测细胞内活性氧（reactive oxygen species，ROS）的水平。方法将新生1天20只乳鼠随机分为3组（试验组15只，阴性和阳性对照组各5只），取乳鼠海马组织制备成单细胞悬液，经蓝莓花色苷、双氧水处理后，加入二氯荧光素一双乙酸盐（DCF-DA）孵育，13（2F-DA进入细胞后可被ROS氧化生成发荧光的2’，7’-二氯荧光素（DCF）52，通过流式细胞仪检测DCF荧光水平的变化，同时设立阴性及阳性对照。结果蓝莓花色苷物质可以降低H2O2刺激诱导的胞内ROS的产生，以1umol／L效果最为明显。结论利用流式细胞仪检测过氧化物刺激后胞内产生的活性氧是一种简单、准确、稳定的方法。Objective To detect the levels of reactive oxygen species （ROS） of hippocampad neurons cells by flow cytometry. Methods Mouses were randomly divided into 3 groups（15 cases in experiment group,5 cases in positive control group and 5 cases in negative control group）. Cells were treated with blueberry anthocyanins and H2O2 before incubating with （DCF- DA）. The fluorescent intensity of cellular DCF was detected by flow cytometry. Negative controls and positive controls were also set up. Results Anthocyanins could reduce intracellular ROS generation induced by H2O2. Conclusion The method by flow eytometry to measure the reactive oxygen species is simple,reliable and stable.

关 键 词：流式细胞术 活性氧 二氯荧光素 双乙酸盐(DCF-DA) 蓝莓花色苷 

分 类 号：R-332[医药卫生]