高糖诱导的视网膜胶质细胞凋亡以及牛磺酸的防护效应研究  

Effect of taurine on high-glucose induced apoptosis of rat retinal glial cells

作　　者：曾凯宏[1] 杨咏涛[1] 王静[1] 余雪梅[1] 崔越[1] 

机构地区：[1]四川省医学科学院.四川省人民医院临床营养科,四川成都610072

出　　处：《成都医学院学报》2012年第1期37-40,共4页Journal of Chengdu Medical College

基　　金：四川省卫生厅资助项目(No.100533)

摘　　要：目的通过检测高糖和牛磺酸培养对大鼠视网膜胶质细胞凋亡的影响,探讨牛磺酸保护糖尿病引起视网膜损伤的机制。方法实验分为10组,分别为:5mmol/L葡萄糖培养组(正常葡萄糖);5mmol/L葡萄糖+(0.1、1和10mmol/L)牛磺酸培养组;25mmol/L葡萄糖培养组(高葡萄糖);25mmol/L葡萄糖+(0.1、1和10mmol/L)牛磺酸培养组;5mmol/L和25mmol/L葡萄糖+20mmol/L甘露醇组。用免疫细胞荧光双标法鉴定胶质细胞,用Annexin-V/PI双染结合流式细胞检测技术检测大鼠视网膜胶质细胞的凋亡情况。结果与正常对照组相比,25 mmol/L高糖组胶质细胞凋亡明显,高糖组的早期凋亡细胞百分率为20.6%;加入0.1、1、10mmol/L牛磺酸干预后,高糖组细胞凋亡率分别降为16.4%、5.7%和7.6%,牛磺酸干预组胶质细胞凋亡率明显低于高糖组(P<0.05)。结论体外高糖培养可诱导大鼠视网膜胶质细胞发生凋亡,而牛磺酸对胶质细胞凋亡具有保护效应。上述结果可为牛磺酸用于糖尿病早期视网膜病变的防治提供重要的实验依据。Objective To investigate the effect of taurine on glial cells apoptosis in rats retina cultured with high glucose and to explore the protective mechanism of taurine against diabetic retinopathy.Methods Cells were cultured in DMEM in the presence of 5 mmol/L D-glucose（normal glucose）,5 mmol/L D-glucose plus 0.1,1 and 10 mmol/L taurine,25 mmol/L D-glucose（high glucose）,25 mmol/L D-glucose plus 0.1,1 and 10 mmol/L taurine,and 5 mmol/L D-glucose（normal glucose）,25 mmol/L D-glucose（high glucose）plus 20 mmol/L mannitol for at least 3 days.Morphology of cells was observed by confocal microscopy.Annexin V/PI staining followed by flow cytometric analysis was used to detect early stages of apoptosis in retinal glial cells of rats cultured with high glucose.Results With 25 mmol/L high glucose incubation,the apoptosis was significantly increased compared with the control.The percentage of early apoptotic cells in high glucose incubation group was 20.6%.When cells were incubated with high glucose and taurine at 0.1,1.0 and 10 mmol/L,the percentage of early apoptosis significantly decreased to 16.4%,5.7% and 7.6%,respectively（all P〈0.05）.Conclusions Our results revealed that taurine ameliorates diabetic retinopathy possibly via decreasing the apoptosis of retinal glial cells,which suggested a potential use of taurine as an adjuvant therapeutic agent in the management of diabetes and its complications.

关 键 词：高糖 视网膜 胶质细胞 凋亡 牛磺酸 

分 类 号：R774.1[医药卫生—眼科]