海洋产几丁质酶菌株的筛选及发酵条件优化  

Studies on the Screening of Chitinase Producing Strain from Ocean and Its Optimization on Fermentation

作　　者：张灿[1] 黄德智[1] 李丰硕[1] 王硕[1] 薛永常[1] 

机构地区：[1]大连工业大学生物工程学院,大连116034

出　　处：《吉林农业大学学报》2012年第2期141-146,共6页Journal of Jilin Agricultural University

基　　金：辽宁省高校创新团队支持计划项目(2008T010)

摘　　要：通过透明圈法初筛、DNS复筛,从大连近海海域的海泥中分离出一株高产几丁质酶的菌株,经初步鉴定为扩展短杆菌(Brevibacterium linens)。采用不同碳源、氮源、温度、pH值等对培养条件进行了优化及正交试验。结果表明:该菌产酶最适条件是在28℃、pH 7.0、蛋白胨1 g/L、胶体几丁质10 g/L、NaCl20g/L条件下,几丁质酶活力可达0.508 U/mL,比优化前其产酶活力提高了9倍。A strain with high productivity of chitinase was screened through transparent circle method from sea mud of Dalian seaside and was identified as species Brevibacterium linens.After medium optimization and orthogonal experiments,which include carbon sources,nitrogen sources,temperature and pH,the chitinase activity was improved.The results showed that the optimized conditions for chitinase production were 1 g/L peptone,10 g/L Colloidal chitin,the NaCl of 20 g/L,culture at 28 ℃and pH 7.0.Under these conditions the chitinase activity can reach 0.508 U/mL,9 times higher than that of parent strain.

关 键 词：几丁质 几丁质酶 菌株筛选 优化 

分 类 号：Q93[生物学—微生物学]