菜籽预榨-浸出粕中植酸的提取  

Extraction of phytic acid from prepress-solvent extracted rapeseed meal

作　　者：鲍传稳[1] 张明辉[1] 刘晔[1] 

机构地区：[1]武汉工业学院化学与环境工程学院,武汉430023

出　　处：《中国油脂》2012年第4期56-59,共4页China Oils and Fats

基　　金：湖北省教育厅科学研究项目(Q20081808)

摘　　要：为实现菜籽粕的综合开发利用,研究了一种从菜籽预榨-浸出粕中两步提取植酸的工艺。考察了酸洗pH、酸洗时间、碱提pH和碱提乙二胺四乙酸二钠(EDTA)浓度等对植酸提取的影响。将经过复合纤维素酶水解的菜籽粕在pH 5.0和50℃下酸洗3 h获得酸洗液,然后用EDTA浓度0.1mol/L的碱性溶液在pH 12.0和50℃下碱提酸沉获得乳清液,经以上两步可将菜籽粕中的植酸基本提取完全。将提取液中的粗植酸经氢氧化钙沉淀并酸化,再经离子交换可获得纯度为69.96%、提取率为58.0%的植酸产品。To realize fully utilization of rapeseed meal, a two - step process for extraction of phytic acid from prepress - solvent extracted rapeseed meal was established. The effects of key parameters on phytic acid extraction, including pH of acid solution, duration of acid solution, pH and ethylene diamine tetraace- tic acid （EDTA） concentration of alkaline solution for extraction were investigated. With an acid solution extracting step at pH 5.0 and 50 ℃ for 3 h and an alkaline solution extracting step at pH 12.0 and 50℃ with the supplement of 0.1 mol/L EDTA followed by acidic precipitation, the phytic acid in cellulase pre- treated rapeseed meal could be almost entirely extracted. The crude phytie acid in extracted solution was purified by calcium hydroxide precipitation and acidification, then it was treated with ion exchange to ob- tain a final product with purity of 69.96% and extraction rate of 58.0%.

关 键 词：菜籽粕 植酸 提取 PH 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程] TS202.3[轻工技术与工程—食品科学与工程]