高效液相色谱法测定镇江香醋中5-羟甲基糠醛的含量  

Determination of 5-hydroxymethylfurfural in Zhenjiang Vinegar by HPLC

作　　者：张玉玉[1] 宋弋[1] 周婷婷[1] 吕茜[1] 杨瑞学[2] 李全宏[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]中国农业机械化科学研究院,北京100083

出　　处：《北京工商大学学报（自然科学版）》2012年第2期39-42,共4页Journal of Beijing Technology and Business University:Natural Science Edition

基　　金：国家"十二五"科技支撑计划项目(2011BAD23B01)

摘　　要：采用高效液相色谱法,对镇江香醋中5-羟甲基糠醛进行了定量分析.样品中的HMF含量的测定条件为:以甲醇-水溶液(体积比为5∶95)为流动相,流速为1.0 mL/min,采用Venusil XBP-C18色谱柱分离,柱温为30℃,UV检测器的检测波长为284 nm.定量分析结果表明,镇江香醋中HMF的含量为8.01 mg/kg.高效液相色谱法分析镇江香醋中HMF含量的方法测定准确,稳定性高,HMF在0.014 3～0.085 8μg的进样范围内线性相关系数为R2=0.999 9,平均回收率98.66%,相对标准偏差为5.53%.A method for determination of 5-hydroxymethylfurfural by high-performance liquid chromatography in Zhenjiang vinegar was developed.Samples were analyzed on a Venusil XBP-C18 column with oven temperature of 30 ℃,using a mixture of methanol and water（5∶95,v/v） as the mobile phase with the flow rate of 1.0 mL/min,and detected at 284 nm.Within the linear range of 0.014 3～0.085 8 μg,the correlation coefficient was 0.999 9.The method was accurate with the average recoveries rate and relative standard deviation of 98.66% and 5.53%,respectively.

关 键 词：镇江香醋 高效液相色谱 5-羟甲基糠醛 含量检测 

分 类 号：O657.72[理学—分析化学]