不同破壁方法对细菌蛋氨酸产量的影响  

Effect of different disintegrated cell wall methods on methionine yieldof bacterium

作　　者：贾翠英[1] 张玉辉[1] 魏娟[1] 

机构地区：[1]河南科技学院生命科学技术学院,河南新乡453003

出　　处：《食品工业科技》2012年第9期325-327,430,共4页Science and Technology of Food Industry

基　　金：河南省科技攻关项目(082102220001)

摘　　要：为考察不同破壁方法对细菌蛋氨酸产量的影响,分别采用碱解、溶菌酶、超声波以及碱解与超声波、溶菌酶与超声波之间的复合破壁方法对一株产蛋氨酸细菌进行细胞破壁,并用氯铵-T法分别测定胞外发酵液和各种破壁条件下胞内蛋氨酸的含量。结果表明:发酵液中蛋氨酸为0.952mg/L,经碱破壁、溶菌酶破壁、超声波破壁、碱与超声破复合破壁、溶菌酶与超声波复合破壁后,蛋氨酸总产量分别提高了10.9%、12.0%、18.3%、19.6%、22.2%,上述结果表明细菌细胞破壁后,蛋氨酸产量明显提高,且不同破壁方法提高程度不同,复合破壁方法与单一破壁方法相比,蛋氨酸产量提高效果显著。Different methods including alkali-hydro,lysozyme,ultrasonic method,alkali-ultrasonic synergic and lysozyme-ultrasonic synergic method were respectively used in order to investigate the effect of different cell wall disrupting methods on methionine yield of bacterium.Ammonium chloride-T method was used to respectively determine the yield of methionine in fermentation broth and in cell.The results showed that in original fermentation broth there was 0.952mg/L of methionine.And after the cell wall being disrupted by different methods such as alkali-hydro,lysozyme,ultrasonic method,alkali-ultrasonic method and lysozyme-ultrasonic method,the total yield of methionine enhanced 10.9%,12.0%,18.3%,19.6% and 22.2% respectively.All of above results improved further that different methods displayed different efficiency for enhancing methionine yield,especially multiple and synergic disrupting method were better than single disrupting method.

关 键 词：蛋氨酸 细胞破壁 蛋氨酸产量 

分 类 号：TS201.24[轻工技术与工程—食品科学]