β-胡萝卜素对人膀胱癌T24细胞生物学行为的影响  

Effects of β-carotene on Biological Behavior of Human Bladder Cancer Cell Line T24

作　　者：吴志平[1] 赵晓昆[2] 吕晨[2] 

机构地区：[1]贵阳医学院附院泌尿外科,贵州贵阳550004 [2]中南大学湘雅二医院泌尿外科,湖南长沙410011

出　　处：《贵阳医学院学报》2012年第2期152-155,共4页Journal of Guiyang Medical College

摘　　要：目的:探讨β-胡萝卜素对人膀胱癌T24细胞细胞增殖和凋亡的影响。方法:不同浓度的β-胡萝卜素作用于T24细胞,MTT法检测细胞增殖,流式细胞术检测T24细胞的凋亡指数。结果:MTT法检测结果显示10μmol/L和20μmol/L组β-胡萝卜素均能明显抑制T24细胞的生长,抑制率分别为28.83%和63.02%(P<0.05);流式细胞术检测显示β-胡萝卜素能诱导T24细胞调亡,5μmol/L组T24细胞的凋亡指数为(0.065±0.018)(P<0.05),10μmol/L和20μmol/L组凋亡指数分别为(0.126±0.022)和(0.190±0.024)(P<0.01),呈现剂量依赖效应,10μmol/L组及20μmol/L组Hochest染色可见凋亡小体形成。结论:β-胡萝卜素抑制T24细胞的生长,并且呈现剂量依赖效应,其机制可能为诱导细胞凋亡。Objective： To explore the effects of β-carotene on proliferation and apoptosis of human bladder cancer cell line T24.Methods： T24 cells were treated with different concentrations of β-carotene for 72 hours.Proliferation of T24 cells was tested with Hochest staining and MTT assay.T24 cell apoptosis was determined with flow cytometry.Results： MTT assay showed that β-carotene at concentrations of 10 μmol/L and 20 μmol/L could significantly inhibit T24 cell proliferation,and the inhibition rates were 28.83% and 63.02% respectively（P0.05）.Flow cytometry showed that β-carotene could induce T24 cell apoptosis,apoptotic index（AI） of 5 μmol/L group was 0.065±0.018（P0.05）,with the increase of β-carotene concentration,AI increased,AI of 10 μmol/L and 20 μmol/L groups were 0.126±0.022 and 0.190±0.024 respectively（P≤0.01）,showing a dose-dependent manner.Apoptotic bodies were detectable when the cells stained by Hochest method.Conclusions： β-carotene could inhibit T24 cell proliferation in a dose-dependent manner,the mechanism may be associated with inducing T24 cell apoptosis.

关 键 词：Β-胡萝卜素 癌 膀胱 肿瘤细胞 培养的 细胞凋亡 流式细胞术 

分 类 号：R73-34[医药卫生—肿瘤]