出芽短梗霉原生质体形成和再生研究  

Research on Formation and Regeneration of Protoplast of Aureobasidium Pullulans

作　　者：靳挺[1] 武玉学[1] 王建平 

机构地区：[1]浙江大学宁波理工学院生物与化学工程分院,浙江宁波315100 [2]浙江省宁波市海洋与渔业研究院,浙江宁波315012

出　　处：《食品与发酵科技》2012年第2期32-35,共4页Food and Fermentation Science & Technology

基　　金：宁波市自然科学基金项目(2010A610026)

摘　　要：本文旨在研究出芽短梗霉原生质体形成和再生的条件。考察了渗透压稳定剂、菌龄、酶解时间、再生培养基等因素对原生质体形成和再生的影响,得到了出芽短梗霉原生质体形成及再生优化工艺条件:渗透压稳定剂为柠檬酸-磷酸缓冲液含0.6mol/L KCl,菌龄43.5h,酶解时间60min,再生培养基为完全培养基含0.5mol/L甘露醇。在此工艺条件下,出芽短梗霉原生质体形成率达99.8%,再生率为20.3%。This paper aimed to study the conditions of protoplast of Aureobasidium pullulans formation and regeneration.The effects of factors such as osmotic stabilizer,cell age,enzyme hydrolysis time and regeneration media were investigated on the formation and regeneration of protoplast of Aureobasidium pullulans.The optimal conditions were as follows： citric acid-phosphoric acid buffer containing 0.6mol/L KCl,cell age of 43.5h,enzyme hydrolysis time of 60min,complete medium containing 0.5mol/L mannitol as regeneration medium.Under the above conditions,the yield of protoplast formation was 99.8%,and the yield of regeneration was 20.3%.

关 键 词：出芽短梗霉 原生质体 形成 再生 

分 类 号：TQ921[轻工技术与工程—发酵工程]