大豆小肽促进益生菌增殖作用的研究  

The function of soybean peptides to promote the growth of probiotics

作　　者：单春乔[1,2] 吴磊 刘秋晨[1] 刘艳 余丽芸[1] 

机构地区：[1]黑龙江八一农垦大学,黑龙江大庆116036 [2]大连三仪药品有限公司,辽宁大连116036

出　　处：《中国微生态学杂志》2012年第4期311-313,317,共4页Chinese Journal of Microecology

摘　　要：本实验复合蛋白酶酶解处理豆粕制备大豆小肽,酶解产物经SephadexG-25凝胶过滤层析,并对其各组分分子量分布及其促进益生菌发酵作用进行了研究,结果显示,大豆蛋白酶解物经SephadexG-25分离后得到了六个肽片段,分子量大部分在1 000 D以下,体外单细菌培养方法测定了大豆小肽对动物肠道常见益生菌的4个菌株(包括粪链球菌、蜡样芽胞杆菌、枯草芽胞杆菌、酵母菌)增殖的影响,结果表明SBP(Soybeanpeptides)能促进这4株细菌的增殖。添加大豆小肽后益生菌Q68与致病菌混合培养实验中,40 h内可以彻底清除金黄色葡萄球菌,48 h内可以完全消灭大肠埃希菌,大豆肽可以促进有益菌的增殖,抑制有害菌的生长。Soybean meals were digested by soybean protease.Then soybean peptides（SBP） were prepared by gel filtration chromatography using Sephadex G-25.And the function of SBP to promote the growth of probiotics was studied.Results showed that the molecular weights of the 6 separated fractions were under 1000D.SBP significantily promoted the growth of common probiotics（Streptococcus faecium cernelle,Cerea Spore Bacilli,Bacillu s subtilis and yeast fungus） in vitro.S.aureus was cleared away within 40 hours and E.coli disappeared within 48 hours in a mixed culture of SBP with one of the 4 probiotics.

关 键 词：大豆小肽 益生菌 促生长 发酵 

分 类 号：S816[农业科学—饲料科学]