Curcumin inhibits proliferation of human lens epithelial cells:a proteomic analysis  

作　　者：Yan-hong HU Xiu-rong HUANG Ming-xin QI Bu-yuan HOU 

机构地区：[1]Department of Ophthalmology,the Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine,Fuzhou 350003,China [2]Research Center of Pathophysiology,Fujian University of Traditional Chinese Medicine,Fuzhou 350003,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2012年第5期402-407,共6页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project(No.wzzb0605) supported by the Traditional Chinese Medicine Research Fund of the Department of Health of Fujian Province,China

摘　　要：Objective: The incidence of after-cataracts [also known as posterior capsular opacification (PCO)] is between 30% and 50% three years following cataract surgery. Suppressing the proliferation of lens epithelial cells (LECs) is a primary goal in preventing PCO. Here, we investigated the proteomic regulation of the inhibitory effects of curcumin (Cur) on the proliferation of human lens epithelial B3 (HLE-B3) cells. Methods: Recombinant human basic fibroblast growth factor (rhbFGF) was used to induce proliferation of HLE-B3 cells, which were incubated with 20 mg/L Cur in a CO2 incubator for 24 h. Results: We found that the absorbance (A) value of rhbFGF group was significantly higher than the A value of the control group. Furthermore, the A value of the Cur group was significantly lower com- pared to the rhbFGF group, with an inhibition of 53.7%. Five different protein spots were obtained from proliferative HLE-B3 cells induced by rhbFGF. Eight different protein spots were obtained in HLE-B3 cells incubated with Cur. There were the common variational protein spots at mass/charge (m/z) ratios of 8 093 and 13 767 between rhbFGF group and control group as well as between the Cur group and rhbFGF group. Conclusions: These results show that Cur effectively inhibited HLE-B3 cell proliferation induced by rhbFGF. The protein spots at m/z of 8 093 and 13 767 may be the targets of Cur-induced inhibition of HLE-B3 cell proliferation. Cur may be a reliable and effective drug for pre- vention and treatment of polymerase chain reaction (PCR).Objective： The incidence of after-cataracts [also known as posterior capsular opacification （PCO）] is between 30% and 50% three years following cataract surgery. Suppressing the proliferation of lens epithelial cells （LECs） is a primary goal in preventing PCO. Here, we investigated the proteomic regulation of the inhibitory effects of curcumin （Cur） on the proliferation of human lens epithelial B3 （HLE-B3） cells. Methods： Recombinant human basic fibroblast growth factor （rhbFGF） was used to induce proliferation of HLE-B3 cells, which were incubated with 20 mg/L Cur in a CO2 incubator for 24 h. Results： We found that the absorbance （A） value of rhbFGF group was significantly higher than the A value of the control group. Furthermore, the A value of the Cur group was significantly lower com- pared to the rhbFGF group, with an inhibition of 53.7%. Five different protein spots were obtained from proliferative HLE-B3 cells induced by rhbFGF. Eight different protein spots were obtained in HLE-B3 cells incubated with Cur. There were the common variational protein spots at mass/charge （m/z） ratios of 8 093 and 13 767 between rhbFGF group and control group as well as between the Cur group and rhbFGF group. Conclusions： These results show that Cur effectively inhibited HLE-B3 cell proliferation induced by rhbFGF. The protein spots at m/z of 8 093 and 13 767 may be the targets of Cur-induced inhibition of HLE-B3 cell proliferation. Cur may be a reliable and effective drug for pre- vention and treatment of polymerase chain reaction （PCR）.

关 键 词：CURCUMIN AFTER-CATARACT Posterior capsular opacification (PCO) PROTEOMICS Lens epithelial cells 

分 类 号：R776.1[医药卫生—眼科]