一株棉秆纤维素分解真菌的分离筛选及酶学性质研究  

Isolation of a Cotton Stalk Cellulose Decomposing Fungus and Its cellulase Properties

作　　者：白宝伟[1] 张琴[1] 滕立平[2] 杨瑛[3] 李艳宾[1] 

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔843300 [2]塔里木大学植物科学学院,新疆阿拉尔843300 [3]塔里木大学机械电气化工程学院,新疆阿拉尔843300

出　　处：《新疆农业科学》2012年第4期701-707,共7页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金(30960071)

摘　　要：【目的】对从棉秆腐解物中筛选得到的高效棉秆分解真菌SJ-1进行纤维素酶酶学性质的研究。【方法】研究温度、pH、金属离子对纤维素酶活力的影响,并以Lineweaver-Burk作图法测定酶促反应米氏常数Km及最大反应速率Vmax。【结果】该菌株CMCase和FPase的最适反应温度在50～60℃,最适pH为7.0,有较好的耐高温及耐碱能力。K+、Fe2+对酶活有显著的激活作用,而Cu2+、Mg2+、Ca2+、Al3+等有一定的抑制作用,其中Al3+的抑制作用较为强烈,Cu2+对FPase抑制较强,Mn2+对FPase有激活作用而对CMCase有抑制作用,Zn2+对酶活性无明显影响。以CMC-Na做底物时酶反应的Km为2.69 mg/mL,Vmax为0.53 mg/(mL.min)。【结论】菌株SJ-1的纤维素酶性质较为优良,为进一步进行菌株的选育与改造提供参考依据。【Objective】The purpose of this project was to study cellulase pr operties of a cellulose decomposing f ungus SJ-1 isolated from cotton stalk decomposing products were studied.【Method】 Effects of temperature,pH value and metal ions on cellulase activity were s tudied,and Michaelis constant Km of enzymatic reaction and the maximum reaction rate Vmax were examined by Lineweaver-Burk method.【Result】The best reaction temperature to CMCase and FPase was ranged from 50 to 60℃,and the best pH va lue was 7.0,which showed the tolerance to high temperature and alkali.K＋ and Fe 2＋ activated the cellulase activity significantly,while Cu2＋,Mg2＋,Ca 2＋ and Al3＋ showed certain inhibition,the inhibition of Al3＋ exhibited much more stro ngly, Cu2＋ inhibited FPase strongly as well,Mn2＋ showed activation to FPase but inhi bition to CMCase,Zn2＋ had no influence on cellulase activity.When CMC-Na was t aken as s ubstra te,Km and Vmax of the enzymatic reaction were 2.69 mg/mL and 0.53 mg/（mL·min） respectively.【Conclusion】The strain showed good properties of cellulase activ ity,and provided references for further breeding and alteration of this fungus.

关 键 词：棉秆 腐解 纤维素分解菌 纤维素酶 酶学性质 

分 类 号：S182[农业科学—农业基础科学] Q936[生物学—微生物学]