甘露糖结合蛋白基因突变与肝硬化及肝癌关系的研究  

Relationship between mannose-binding protein polymorphism and patients with liver cirrhosis and hepatocellular carcinoma

作　　者：郑瑞丹[1] 庄群瑛[1,2] 高建平[3] 卢燕辉[1] 陈建能[1] 陈洁[1] 朱青川 林震群 

机构地区：[1]厦门大学附属东南医院肝病治疗中心,漳州363000 [2]厦门大学公共卫生学院08级预防医学系,厦门361005 [3]福建省漳州市中医院检验科,漳州363000

出　　处：《中国肝脏病杂志（电子版）》2012年第1期1-4,共4页Chinese Journal of Liver Diseases：Electronic Version

基　　金：福建省漳州市科技计划资助项目(Z2010085)

摘　　要：目的探讨甘露糖结合蛋白(MBP)基因突变与肝硬化及肝癌的关系。方法采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法和实时荧光定量PCR(FQ-PCR)技术针对代偿性肝硬化(CC)患者73例、失代偿性肝硬化(DC)患者78例、肝细胞癌(HCC)患者35例和对照组88例健康者的MBP基因第54位密码子多态性进行检测。结果 HCC组的MBP基因GGC/GAC基因型频率和GAC等位基因频率与对照组比较,差异无统计学意义(P>0.05)。CC组、DC组MBP基因GGC/GAC基因型频率和GAC等位基因频率均显著高于HCC组和对照组(P<0.05),其中DC组突变率最高(36.5%)。结论 MBP第54位密码子与肝硬化进展相关,但可能并非HCC发展中的重要因素。Objective To explore the relationship between mannose-binding protein （MBP） codon 54 polymorphism and patients with liver cirrhosis and hepatocellular carcinoma. Methods Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and fluorescent quantitative PCR （FQ-PCR） were conducted on the MBP codon 54 polymorphism in 73 patients with compensated cirrhosis （CC）, 78 with decompensated cirrhosis （DC）, 35 with hepatocellular carcinoma （HCC） and 88 normal controls. Results The genotype frequency of GGC/GAC heterozygotes and GAC allele frequency were significantly higher in group CC and DC as compared with that of control group and HCC group （P 0.05）, while no difference was found between HCC group and normal subjects （P 0.05）. GAC allele frequency was also highest prevalence （36.5%） in DC group than that in CC group and HCC group （P 0.05）. Conclusions The MBP codon 54 polymorphism is associated with the progression of liver cirrhosis and might not play an important role in the development of hepatocellular carcinoma.

关 键 词：甘露糖 肝硬化 肝肿瘤 密码子 

分 类 号：R575.2[医药卫生—消化系统]