胡萝卜果胶的化学结构特征研究与含量测定  

Chemical Structure and Content Determination of Carrot Pectin

作　　者：李洋[1] 何立巍[1] 何睦[1] 李晓曦[1] 彭稳稳[1] 笪婧雯[1] 耿婷婷[1] 武圆圆[1] 

机构地区：[1]南京中医药大学,南京210046

出　　处：《中国药房》2012年第19期1779-1781,共3页China Pharmacy

摘　　要：目的:研究胡萝卜果胶的化学结构特征,并测定其含量。方法:采用高效凝胶渗透液相色谱法测定胡萝卜果胶的相对分子质量;纸色谱、高效液相色谱法鉴别果胶中主要单糖组成;紫外、红外光谱法鉴别其化学结构特征;硫酸-咔唑法测定果胶的含量。结果:胡萝卜果胶的平均相对分子质量为1750625,主要单糖组成为D-半乳糖和D-半乳糖醛酸;单糖的连接方式主要为β-吡喃型糖苷键连接。果胶含量以半乳糖醛酸计为66.06%。结论:胡萝卜果胶提取物多糖含量较高、化学结构特征明显。本试验结果可为胡萝卜果胶的进一步开发提供理论和试验基础。OBJECTIVE：To study the chemical structure of carrot pectin and to determine the content of it.METHODS：The average molecular weight was determinate by HPGPC;Paper chromatography （PC） and HPLC were used to identify the major monosaccharide component of pectin;UV and IR spectroscopy indentify the chemical structure;the content of pectin was determined by sulfuric acid-cardyl method.RESULTS：The average molecular weight of carrot pectin was 1 750 625 and monosaccharide mainly composed of D-galactolipin and D-galacturonic acid;monosaccharide was mainly connected by β-pyran type glycosidic bonds.The content of pectin to galacturonic acid was 66.06%.CONCLUSION：The carrot pectin extract has large content of polysaccharide and obvious chemical structure.The trial provides theory and trial basis for further development of carrot pectin.

关 键 词：胡萝卜 果胶 含量测定 化学结构 

分 类 号：R284.1[医药卫生—中药学] R927[医药卫生—中医学]