蒸汽爆破预处理玉米芯及其酶解工艺研究  

Steam explosion pretreatment and zymohydrolysis of corn cobs

作　　者：任天宝[1,2] 邱盼[2] 任秀娟[1] 张安邦[1] 杨渤 张东升 

机构地区：[1]河南科技学院资源与环境学院,河南新乡453003 [2]河南农业大学农业部农村可再生能源重点实验室,河南郑州450002 [3]鹤壁互邦农业科技有限公司,河南鹤壁458000 [4]国家粮油及肉制品质量监督检验中心,河南漯河462000

出　　处：《可再生能源》2012年第5期59-63,共5页Renewable Energy Resources

基　　金：国家自然科学基金资助项目(70741032);国家农业成果转化资金项目(2006GB2D000173)

摘　　要：以蒸汽爆破预处理后的玉米芯为原料,进行了玉米芯酶解工艺条件的研究。粉碎后的玉米芯在压力2.8 MPa、保压时间240 s条件下蒸汽爆破预处理,在初始固形物含量为14%(w/v),pH 5.0的条件下,分别添加纤维素酶15 FPA/g(底物)、木聚糖酶225 IU/g(底物),同时添加环境因子MgSO40.005 g/g(底物)、Tween-800.001 g/g(底物),糖化48 h后,还原糖浓度达到71.81 g/L,糖化率达到80.85%。试验结果表明,蒸汽爆破预处理及添加适量环境因子对玉米芯的糖化效果影响显著。In this study,Utilizing corn cobs after steam explosion as material to research on enzymatic hydrolysis of corn cobs.The results showed,maintenance pressure time 240 s and steam pressure 2.8 MPa,the initial ratio of solid content was 14%（W/V）,added cellulase（15 FPA/g,zymolyte）,xylanase（225 IU/g,zymolyte） under pH 5.0 respectively.Simultaneously the environmental factors（MgSO4 0.005 g/g and Tween-80 0.001 g/g） were added.After 48 hours,the final concentration of reducing sugars was 71.81 g/L and saccharification rate reached 80.85%.This study demonstrated that the steam explosion pretreatment and proper amount of environmental factors could significantly improve the reducing sugars of corn cobs.

关 键 词：玉米芯 蒸汽爆破 预处理 酶解 

分 类 号：TK6[动力工程及工程热物理—生物能] S216.2[农业科学—农业机械化工程]