枯草芽孢杆菌木聚糖酶Xyn B在毕赤酵母中的表达  

Expression of Bacillus subtilis strain xylanse B gene in Pichia pastouris

作　　者：莫毅 梁方方[2] 赖志强[2] 卢玉发[2] 廖玉英[2] 何仁春[2] 黄丽霞[2] 杨琳[3] 

机构地区：[1]广西人口和计划生育研究中心 [2]广西畜牧研究所 [3]华南农业大学动物科学学院

出　　处：《中国饲料》2012年第9期19-21,共3页China Feed

基　　金：广西青年基金项目资助(桂科青0991068)

摘　　要：编码枯草芽孢杆菌(Bacillus subtilis strain GD3b)木聚糖酶(Xylanse B,Xyn B)基因从重组载体pUC-XynB中切割,并克隆到pPICZa A载体中,经酶切和测序鉴定,成功构建了分泌型毕赤酵母表达载体pPICZa-Xyn B。载体经线性化后转入毕赤酵母X-33,并筛选获得可通过甲醇诱导分泌表达Xyn B重组蛋白的毕赤酵母工程菌X-33/Xyn B。The Bacillus subtilis strain xylanse B（Xyn B）gene was digested from the recombinant plasmid pUC-Xyn B and cloned into vector pPICZa A.After restriction enzyme digestion and sequencing identifications,the secreted expression pichia vector pPICZa-Xyn B was constructed successfully.The recombinant vector pPICZa-Xyn B was linearized and transformed into Pichia pastoris X-33 to rebuild the Pichia pastoris recombinant strain X-33/Xyn B,which could be secreted express Xyn B recombinant fusion protein with methanol induction.

关 键 词：木聚糖酶 毕赤酵母 表达 重组蛋白 

分 类 号：S816.7[农业科学—饲料科学]