响应曲面法优化仲丁胺气相色谱检测的衍生条件  

Optimization of Derivatization Conditions for Gas Chromatographic Determination of Sec-Butylamine by Response Surface Methodology

作　　者：纪淑娟[1] 于兰洁[1] 刘玲[1] 白冰[1] 周丹[1] 

机构地区：[1]沈阳农业大学食品学院,辽宁沈阳110866

出　　处：《食品科学》2012年第8期211-215,共5页Food Science

基　　金：农业部行业标准制定项目(2130109)

摘　　要：利用响应面法优化仲丁胺气相色谱检测的衍生条件。根据Box-Behnken中心组合试验设计原理,针对试验中衍生化反应这一重要环节,在单因素试验基础上,以仲丁胺回收率为响应值,应用响应曲面法研究衍生反应时间、温度、衍生剂用量各因素间的交互作用及其对仲丁胺测定回收率的影响。以五氟苯甲醛溶液作为衍生剂,经气相色谱电子捕获检测器分析测定。确定最佳衍生条件为:衍生反应时间31.72min、衍生反应温度22.42℃、衍生剂用量1.25mL。Derivatization reaction is very important for the determination of sec-butylamine.For the purpose of establishing a gas chromatography-electron capture detection（GC-ECD） method for determining sec-butylamine,the optimal derivatization reaction conditions were studied by response surface methodology.Based on the results of one-factor-at-a-time experiments,a central composite design was used to investigate the pairwise interaction effects of reaction time,temperature and derivatization reagent（acetonitrile containing 0.05μL/mL pentafluorobenzaldehyde） amount on sec-butylamine recovery.The optimal derivative temperature,derivative duration and derivatization reagent amount were found to be 22.42 ℃,31.72 min and 1.25 mL,respectively.

关 键 词：仲丁胺 残留 衍生 响应曲面法 气相色谱 

分 类 号：TS207.3[轻工技术与工程—食品科学]