改性甘薯果胶对癌细胞增殖的影响  

Effects of Modified Sweet Potato Pectins on the Proliferation of Cancer Cells

作　　者：张燕燕[1] 木泰华[1] 张苗[1] 

机构地区：[1]中国农业科学院农产品加工研究所果蔬加工研究室,北京100193

出　　处：《中国农业科学》2012年第9期1798-1806,共9页Scientia Agricultura Sinica

基　　金：农业部现代农业产业技术体系建设专项(CARS-11-B-19);国际科技合作计划项目(2010DFA32690)

摘　　要：【目的】探讨pH改性和热改性甘薯果胶对结肠癌细胞HT-29、乳腺癌细胞Bcap-37和肝癌细胞SMMC-7721增殖的影响。【方法】分别对改性前后甘薯果胶的半乳糖醛酸含量、酯化度、分子量、微观结构及癌细胞增殖抑制活性进行测定。【结果】果胶改性后半乳糖醛酸含量显著提高(P<0.05),而酯化度和分子量降低,微观结构发生明显变化。未改性、pH改性和热改性甘薯果胶对3种癌细胞均有抑制作用,并呈浓度和时间依赖性;改性后甘薯果胶对3种癌细胞增殖抑制效果均有显著提高(P<0.05),且改性甘薯果胶对HT-29和Bcap-37的抑制效果更显著。【结论】改性甘薯果胶对HT-29和Bcap-37的增殖抑制效果较好,具有潜在的抗结肠癌和乳腺癌作用。[ Objective ] Effects of pH-modified and heat-treated sweet potato pectins on proliferation of human colon cancer cell HT-29, human breast cancer cell Bcap-37 and human hepatoma cancer cell SMMC-7721 were investigated. [ Method] The galacturonic acid content, degree of esterification （DE）, molecular weight, microstructure and activity of cancer cell proliferation inhibition of pectins （natural, pH-modified and heat-treated pectins） were determined, respectively. [Result] Modification increased galacturonic acid content of sweet potato pectin, whereas decreased its DE and molecular weight significantly （P〈0.05）. The microstructures of modified pectins were obviously different from microstructure of natural pectin. Natural and modified sweet potato pectins could inhibit the proliferation of three types of cancer cells in time- and manner. In addition, the inhibitory effect of modified sweet potato pectins on three types of cancer cells increased significantly compared to natural pectin （P〈0.05）. Furthermore, the proliferation-inhibitory effects of modified pectins on HT-29 and Bcap-37 were better than that of modified pectins on SMMC-7721. [Conclusion] Proliferation of HT-29 and Bcap-37 could be inhibited effectively by modified sweet potato pectins. It is, therefore, suggested that modified sweet potato pectins have potential applications in prevention of human colon cancer and breast cancer.

关 键 词：甘薯果胶 改性 癌细胞 增殖 

分 类 号：R151[医药卫生—营养与食品卫生学] S531[医药卫生—公共卫生与预防医学]