在线预处理对乙二胺四乙酸二钠离子色谱分离的影响及其应用  

Determination of Disodium Ethylene Diaminetetraacetate in Samples Based on Ion Chromatography with In Guard H Column

作　　者：张婷婷 潘广文 胡忠阳 叶明立 

机构地区：[1]赛默飞世尔科技上海实验室,上海201203

出　　处：《分析化学》2012年第5期813-814,共2页Chinese Journal of Analytical Chemistry

摘　　要：1引言 乙二胺四乙酸二钠（EDTA-2Na）作为食品添加剂,能够对原料中的微量金属离子进行络和,防止金属离子对产品质量的缓慢破坏作用[1],常用在果蔬罐头、八宝粥等食品中,同时也可作为蛋白洗涤液中的添加剂,既增加舒适度又提高溶液杀菌能力。但是含量过高,会刺激皮肤和粘膜,引起哮喘等疾病,A method for the determination of EDTA was developed and validated based on ion chromatography with suppressed conductimetric detection and anion exchange column.The pre-concentration of EDTA was obtained on In guard H by using pump and ten ports valve,then the optimization separation condition was performed on a IonPac AS22（250 mm×4.0 mm） using 4.5 mmol/L carbonate and 1.4 mmol/L bicarbonate buffer solution with 18% acetonitrile as the mobile phase with Isocratic elution.Good resolution was achieved from commonly coexisting anions and organic acid.The interference of different metal cations（Ca2＋,Mg2＋,Cu2＋,Zn2＋ and Fe3＋） and the increase of molar ratio were also examined.The ion chromatographic method was applied for the assay of EDTA in contact lens care solutions、salad and canned foods with simple treatment.The linear range of EDTA was 0.6-50 mg/kg.The recovery of EDTA was about 91.2%-97.0%.

关 键 词：ION EXCHANGE CHROMATOGRAPHY DISODIUM ETHYLENE diaminetetraacetate 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.7[轻工技术与工程—食品科学与工程]