去卵巢骨质疏松模型大鼠的番茄红素干预(英文)  

Antiosteoporotic activity of lycopene in mature ovariectomized rats

作　　者：杨希重[1] 季翠杰[2] 王德春[1] 陈晓亮[1] 

机构地区：[1]青岛大学医学院附属医院脊柱外科,山东省青岛市266003 [2]即墨市人民医院神经内科,山东省即墨市266200

出　　处：《中国组织工程研究》2012年第15期2750-2756,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:有研究表明番茄红素作为一种抗氧化物质能够减低患慢性疾病的风险。目的:建立去卵巢骨质疏松大鼠模型,通过给去卵巢大鼠灌服不同剂量的番茄红素,观察其对实验性骨质疏松的保护作用。方法:切除6月龄SPF级雌性Wistar未生育大鼠双侧卵巢1周,建立去卵巢骨质疏松大鼠模型,分别给予苯甲酸雌二醇,番茄红素10mg,20mg进行干预。结果与结论:各组给药12周后,番茄红素高低剂量组与卵巢切除组比较子宫质量,血清中的钙、磷含量,骨小梁面积百分比,骨小梁数目增加,血清中超氧化物歧化酶活性,骨密度和骨生物学性能均明显增高(P<0.05);血清中碱性磷酸酶,尿中脱氧吡啶啉,丙二醛,骨小梁分离度和破骨细胞数均减少(P<0.05)。结果证实,番茄红素能明显缓解去卵巢引起的子宫萎缩,清除体内的自由基,改善去卵巢大鼠的氧化应激状态,减少骨吸收增加骨形成。BACKGROUND：A number of studies have suggested that lycopene is an antioxidant which is used to decrease the risk of age-related chronic diseases.OBJECTIVE：To establish a rat model of postmenopausal osteoporosis,and to investigate the effects of lycopene in preventing bone loss in ovatiectomized rat models of osteoporosis.METHODS：The 6-month-old female Wistar rats,SPF grade,which had never given birth,were divided into sham and ovatiectomized groups.The ovatiectomized rats were further sub-divided into four groups which were administered with corn oil orally as model group,lycopene（10 mg/kg,20 mg/kg,daily） or treated by intraperitoneal injection with estradiol benzoate（25 mg/kg,twice）.RESULTS AND CONCLUSION：After 12-week administration,as compared with the model group,the uterus weight of high-dose and low-dose lycopene groups significantly increased（P 0.05）;the Ca,P,superoxide dismutase levels,percent trabecular area,and trabecular number were obviously higher,and serum alkaline phosphatase,urine deoxypyridinoline,malondialdehyde,trabecular separation and number of osteoclasts were obviously lower in the lycopene groups than the model group（P 0.05）.The bone mass index,bone bio-mechanics and bone histomorphometry were better in the lycopene groups than the model group（P 0.05）.These findings indicate that lycopene has a definite antiosteoporotic effect on ovatiectomized rats.

关 键 词：番茄红素 去卵巢 氧化应激 骨质疏松 大鼠 骨组织构建 

分 类 号：R318[医药卫生—生物医学工程]