广州流动儿童病毒性腹泻监测  

The surveillance of viral diarrhea among migrant children in Guangzhou

作　　者：冀新凤[1] 罗灿[2] 胡婷婷[1] 罗敏红[1] 李美青[1] 刘颖[3] 陈清[1] 俞守义[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院,广东广州510515 [2]广东省妇幼保健院,广东广州510010 [3]广州市皮肤病防治所,广东广州510095

出　　处：《热带医学杂志》2012年第4期418-421,共4页Journal of Tropical Medicine

摘　　要：目的了解广州流动儿童病毒性腹泻的感染情况。方法收集2010年5月至2011年4月广州市白云区某社区卫生服务站241例流动儿童腹泻患者的人口资料学以及粪便标本,利用酶联免疫技术(ELISA)检测轮状病毒(RV),采用逆转录聚合酶链反应(RT-PCR)法检测标本中诺如病毒(NVs)、星状病毒(AstV),聚合酶链反应(PCR)法检测腺病毒(AdV)。结果 241例患者粪便标本中RV、NVs、AdV、AstV4种病毒总检出率为56.0%(135/241),各病毒检出率分别为43.6%(105/241)、11.2%(27/241)、7.9%(19/241)、6.6%(16/241)。27株NVs阳性毒株均为GII-4;19株AdV中14株为AdV-41型,并有AdV1、2、3、31型的散在感染;AstV感染15株,均为AstV-1型。结论病毒为流动儿童腹泻的重要病因,其中轮状病毒是最主要的病原体,其他依次为NVs、AdV和AstV。Objective To understand the status of viral diarrhea among migrant children in Guangzhou. Methods Fecal specimens and demographic information were collected from 241 patients of migrant children with diarrhea from May 2010 to April 2011. Fecal samples were determined to identify rotavirus（RV） by ELISA and norovirus（NVs）, astrovirus （AstV） by RT-PCR and adenovirus（AdV） by PCR. Results At least one of the four viral agents was found in 56.0% of the specimens. Rotavirus,norovirus,adenovirus and astrovirus were identified in 43.6%,11.2%,7.9%,6.6% of the 241 specimens,respectively..All 27 NVs sequenced strains belonged to GII-4. Fourteen of 19 AdV sequenced strains were AdV-41 serotype..Sporadic infection of AdV-1,AdV-2,AdV-3 and AdV-31 serotype were also found..Fifteen samples were positive for AstV which belonged to AstV-1 serotype. Conclusions Viral agents had the important impact causing diarrhea among migrant children in Guangzhou. RV is the dominant etiological agent and NVs,AdV and AstV are also the important etiological agent of migrant children diarrhea in Guangzhou.

关 键 词：病毒 腹泻 流动儿童 监测 

分 类 号：R574.62[医药卫生—消化系统]