Proteomic profiling of lipid droplet-associated proteins in primary adipocytes of normal and obese mouse  

作　　者：Yubo Ding Yibo Wu Rong Zeng Kan Liao 

机构地区：[1]State Key Laboratory of Cell Biology,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Graduate School,Chinese Academy of Sciences,Shanghai 200031,China [2]Key Laboratory-of Systems Biology~Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China [3]present address:Institute of Molecular Systems Biology,Swiss Federal Institute of Technology Zurich,ETH Hönggerberg,CH-8093 Zürich,Switzerland

出　　处：《Acta Biochimica et Biophysica Sinica》2012年第5期394-406,共13页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the Ministry of Science and Technology of China （2010CB912102）, from the National Natural Science Foundation of China （30821065 and 30870559）.

摘　　要：Lipid droplets in adipocytes serve as the principal long- term energy storage depot of animals. There is increasing recognition that lipid droplets are not merely a static neutral lipid storage site, but in fact dynamic and multi- functional organeiles. Structurally, lipid droplet consists of a neutral lipid core surrounded by a phospholipid mono- layer and proteins embedded in or bound to the phospho- lipid layer. Proteins on the surface of lipid droplets are crucial to droplet structure and dynamics. To understand the lipid droplet-associated proteome of primary adipocyte with a large central lipid droplet, lipid droplets of white adipose tissue from C57BL/6 mice were isolated. And the proteins were extracted and analyzed by liquid chromatog- raphy coupled with tandem mass spectrometry. A total of 193 proteins including 73 previously unreported proteins were identified. Furthermore, the isotope-coded affinity tags （ICAT） was used to compare the difference of lipid droplet-associated proteomes between the normal lean and the high-fat diet-induced obese C57BL/6 mice. Of 23 proteins quantified by ICAT analysis, 3 proteins were up- regulated and 4 proteins were down-regulated in the lipid droplets of adipose tissue from the obese mice. Importantly, two structural proteins of lipid droplets, perilipin A and vimentin, were greatly reduced in the lipid droplets of the adipose tissue from the obese mice, implicat- ing reduced protein machinery for lipid droplet stability.Lipid droplets in adipocytes serve as the principal long- term energy storage depot of animals. There is increasing recognition that lipid droplets are not merely a static neutral lipid storage site, but in fact dynamic and multi- functional organeiles. Structurally, lipid droplet consists of a neutral lipid core surrounded by a phospholipid mono- layer and proteins embedded in or bound to the phospho- lipid layer. Proteins on the surface of lipid droplets are crucial to droplet structure and dynamics. To understand the lipid droplet-associated proteome of primary adipocyte with a large central lipid droplet, lipid droplets of white adipose tissue from C57BL/6 mice were isolated. And the proteins were extracted and analyzed by liquid chromatog- raphy coupled with tandem mass spectrometry. A total of 193 proteins including 73 previously unreported proteins were identified. Furthermore, the isotope-coded affinity tags （ICAT） was used to compare the difference of lipid droplet-associated proteomes between the normal lean and the high-fat diet-induced obese C57BL/6 mice. Of 23 proteins quantified by ICAT analysis, 3 proteins were up- regulated and 4 proteins were down-regulated in the lipid droplets of adipose tissue from the obese mice. Importantly, two structural proteins of lipid droplets, perilipin A and vimentin, were greatly reduced in the lipid droplets of the adipose tissue from the obese mice, implicat- ing reduced protein machinery for lipid droplet stability.

关 键 词：PROTEOMICS OBESITY lipid droplet-associatedprotein primary adipocyte 

分 类 号：Q51[生物学—生物化学] S828.1[农业科学—畜牧学]