古尼拟青霉麦角甾醇高产液体培养条件及提取工艺的优化  

Optimization of liquid culture conditions and extraction process of ergosterols from Paecilomyces gunnii

作　　者：蒋岚[1] 于士军[1] 张莹[1] 张琛[1] 樊美珍[1] 李春如[1] 

机构地区：[1]安徽农业大学安徽省微生物防治重点实验室,安徽合肥230036

出　　处：《菌物学报》2012年第3期413-421,共9页Mycosystema

摘　　要：以古尼拟青霉生物量和麦角甾醇含量为指标,对古尼拟青霉的高产麦角甾醇培养条件及麦角甾醇提取工艺进行了优化。正交试验结果表明,高产麦角甾醇的最适液体培养基为蔗糖4%,麸皮2%,KH2PO40.025%和NaCl0.05%;响应面实验结果表明,麦角甾醇最佳提取工艺为:提取溶剂浓度为80%乙醇,液料比为200:1,提取时间为65min。Liquid culture conditions and extraction process of ergosterols from the entomopathogenic fungus Paecilomyces gunnii were optimized by using the indices of biomass and ergosterol productions. The result of orthogonal test indicated that the optimal liquid medium contained sucrose 4%, bran 2%, KH2PO4 0.025% and NaC1 0.05%. Response surface experiment demonstrated that the optimal extract solvent concentration was 80% ethanol, the ratio of solvent to raw material was 200：1 and the extract duration was 65min.

关 键 词：古尼拟青霉 昆虫病原真菌 麦角甾醇 最适液体培养基 最适培养条件 

分 类 号：TQ929[轻工技术与工程—发酵工程]