原发性肝癌8号和16号染色体杂合子丢失的研究  

Ioss of heterozygosity on chromosomes 8 and 16 in primary hepatocellular carcinoma

作　　者：周后龙 巩丽[2] 张伟[2] 杜云翔 张建宇 冯英明[3] 

机构地区：[1]解放军第八二医院肿瘤科,江苏淮安223001 [2]第四军医大学唐都医院病理科,陕西西安710038 [3]第四军医大学唐都医院肿瘤科,陕西西安710038

出　　处：《现代肿瘤医学》2012年第6期1134-1138,共5页Journal of Modern Oncology

摘　　要：目的:分析人肝细胞肝癌(HCC)组织中染色体8和16部分染色体片段的杂合子丢失及与临床病理关系,初步筛选HCC相关的抑癌基因,为HCC的早期诊断、预后预警提供可能的新分子标记物。方法:应用聚合酶链反应-变性聚丙烯酰胺凝胶-银染法分析45例HCC组织标本中分别位于染色体8和16上的具有高度多态性微卫星位点的杂合性丢失(LOH)状态。结果:发生LOH的总频率为68.89%(31/45),其中D16S511位点的LOH发生率最高为53.33%(24/45),其次是D8S261(39.02%,16/41)和D8S499(34.88%,15/43)。结论:染色体16q23、8p22-21.3及8p12区域的LOH发生频率高,可能存在与HCC发生发展相关的新的抑癌基因,特定位点的遗传变异可能与HBV感染、临床病理恶性程度等预后因素相关。Objective：To study the loss of heterozygosity on chromosomes 8p and 16q in primary hepatocellular carcinoma（HCC）,evaluate the relationship between the loss and clinicopathologic features and try to screened some HCC-related tumor suppressor genes in order to provide possible molecular markers for early diagnose and prognosis warning of HCC screening.Methods： The frequency of loss of heterozygosity（LOH） at some specific microsatellite loci on chromosome 8p and 16q in tissue samples from thirty-five patients with HCC were examined by using PCR-denaturing PAGE-silver staining.Results： The overall LOH frequency was（68.89%,31/45） at least one locus of 8 loci on the chromosomes.The three most more frequent loci were D16S511（53.33%,31/45）,D8S261（39.02%,16/41）and D8S499（34.88%,15/43）.Conclusion： There may be a new putative tumor suppressor gene related to the occurrence and development on specific chromosome region 16q23,8p22-21.3 or 8p12 with high-frequent LOH.The genetic alterations on some specific loci were associated with such prognosis factors as the positive HBsAg,differentiated degrees of HCC.

关 键 词：肝细胞肝癌 杂合性丢失 染色体 

分 类 号：R735.7[医药卫生—肿瘤]