机构地区:[1]云南农业大学,云南省动物营养与饲料重点实验室,云南昆明650201
出 处:《云南农业大学学报(自然科学版)》2012年第3期346-352,共7页Journal of Yunnan Agricultural University:Natural Science
基 金:云南省自然科学基金重点项目(2005C0008Z);云南省自然科学基金项目(2011FB052)
摘 要:为研究日粮不同能量和蛋白质水平对乌金猪肌肉组织能量代谢的影响,试验选取体重约15 kg的乌金猪108头,随机分为6组,下设3个重复,每个重复6头,在15~30,>30~60,>60~100 kg体重阶段分别饲喂不同能量和蛋白质水平日粮,能量水平为:高消化能(HE,14.22 MJ/kg)、中消化能(ME,12.89 MJ/kg)和低消化能(LE,11.74 MJ/kg),蛋白水平为:高蛋白质(HP,18%,16%,14%)、中蛋白质(MP,16%,14%,12%)和低蛋白质(LP,14%,12%和10%),在体重30,60,100 kg体重时分批屠宰,取血液、肝脏及肌肉组织样品,测定血糖、肝糖原、肌糖原和肌肉中乳酸的含量及肌肉中乳酸脱氢酶、钠钾ATP酶(Na+K+-ATP酶)和钙镁ATP酶(Ca2+Mg2+-ATP酶)的活性。结果表明,乌金猪血糖、肝糖原、肌糖原和肌肉中乳酸的含量及肌肉中乳酸脱氢酶、Na+K+-ATP酶和Ca2+Mg2+-ATP酶的活性随日粮能量和蛋白质水平的升高而升高;在60 kg体重时,高能量和高蛋白质日粮组乌金猪上述各项指标均明显升高(P<0.05),在100 kg体重时,高能量和高蛋白质日粮组乌金猪血糖、肝糖原和肌糖原的含量及Na+K+-ATP酶和Ca2+Mg2+-ATP酶的活性明显高于低能量和低蛋白质组(P<0.05)。本次试验结果表明高能量和高蛋白质日粮(HE,14.22 MJ/kg和HP,18%)可促进乌金猪肝糖原和肌糖原的合成,肌肉组织中糖酵解途径供能增加,能量代谢加强。研究结果对阐明日粮不同营养水平对猪肌肉组织能量代谢的影响机制具有重要科学意义。The aim of the study was to investigate the effects of dietary energy and protein levels on energy metabolism of muscle tissue in Wujin pigs. One hundred and eight Wujin pigs were randomly allotted into six groups fed diet containing different energy or protein levels. There were eighteen pigs in each group with three replicates which contained six pigs for each replicate. The dietary energy and protein levels were high energy ( HE, 14.22 MJ/kg), medium energy ( ME, 12. 89 MJ/kg) and low energy (LE, 11. 74 MJ/kg), high protein (HP, 18%, 16% and 14%), medium protein (MP, 16%, 14% and 12% ), low protein (LP, 14%, 12% and 10% ) respectively. At the body weight of 30, 60 and 100 kg, Wujin pigs were slaughtered and blood, liver and muscular tissues were taken for analyzing contents of blood sugar, hepatic glycogen, muscle glycogen, lactic acid, and activity of lactate dehydrogenase, Na^+ K^ +-ATPase and Ca^2 + Mg^2 +-ATPase. The results showed that contents of blood sugar, hepatic glycogen, muscle glycogen and lactic acid and the activity of Na ^+ K ^+-ATPase, Ca^2+ Mg^2+-ATPase and lactate dehydrogenase increased as Wujin pigs fed dietary of energy or protein level increased. The higher dietary energy or protein level could increase the contents of blood sugar, hepatic glycogen, muscle glycogen and lactic acid and the activity of Na ^+ K ^+-ATPase, Ca^2 + Mg^2 +-ATPas, and lactate dehydrogenase at 60kg body weight (P 〈 0. 05). At the body weight of 100 kg, the higher dietary energy or protein level could increase contents of blood sugar, hepatic glycogen and muscle glycogen and the activity of Na^ + K ^+-ATPase and Ca^2+ Mg^2 +-ATPase. The results indicated that higher energy ( HE, 14. 22 MJ/kg) or protein ( HP, 18% ) level of dietary could promote synthesis of hepatic glycogen and muscle glycogen, increase the action of glycolytic pathway, and therefore advanced the energy metabolism of muscle tissue in Wujin pigs.
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