利用紫外光谱研究麦冬蛋白质提取分离的优化条件  

Conditions Optimization on Extraction Separation of Ophiopogon japonicus Protein

作　　者：梁书凤[1] 成兰英[1] 张治强[1] 

机构地区：[1]西南科技大学材料科学与工程学院,四川绵阳621010

出　　处：《广州化工》2012年第9期72-74,共3页GuangZhou Chemical Industry

基　　金：四川省教育厅重点项目(NO.07Zd1117);博士研究基金项目(NO.07ZX0108)

摘　　要：采用4℃下以不同的料液比、提取时间、磷酸盐缓冲溶液pH、乙醇溶液浓度、硫酸铵溶液浓度,进行单因素试验,对麦冬蛋白质浸提,测定蛋白质提取液的吸光度,确定了麦冬蛋白质提取分离的最优条件。研究结果表明:提取分离的优化条件是以料液比1:20,pH 9.00的磷酸盐缓冲溶液提取6 h。Optimal conditions of extraction separation of Ophiopogon japonicus protein were studied by signal factor experiments of extracting protein under 4℃, including solid - liquid ratio, extracting time, pH of sodium phosphate buff- er, concentration of ethanol and ammonium sulfate, and the absorbance of extracting solution was determined. Results showed that the optimal separation conditions were extracting for 6 h at pH 9.00 in sodium phosphate buffer with the solid - liquid ratio of 1 ： 20, respectively.

关 键 词：麦冬 蛋白质 提取分离 条件优化 

分 类 号：Q946.1[生物学—植物学]