应用正交试验法优选茎瘤芥高效遗传转化体系  

Optimization of Genetic Transformation System of Tumorous Stem Mastard by Orthogonal Design

作　　者：梁婷[1] 刘毅[2] 蔡应繁[2] 岳显可[1] 江怀仲[2] 何晓红[2] 

机构地区：[1]成都中医药大学研究生院,四川成都611130 [2]重庆邮电大学生物信息学院,四川重庆400065

出　　处：《安徽农业科学》2012年第14期8031-8034,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(31071461;30771311);重庆市自然科学基金(cstc2007BB1328)

摘　　要：[目的]建立茎瘤芥高效遗传转化体系,为茎瘤芥及芸薹属植物遗传转化奠定基础。[方法]应用正交试验法研究茎瘤芥遗传转化过程中预培养时间、共培养时间、侵染液浓度、侵染时间、对转化率的影响。[结果]最适合茎瘤芥CRY2基因RNAi载体遗传转化的条件为预培养3 d、共培养4 d、侵染液浓度OD600=0.5、侵染10 min,此时Kana选择压为30 mg/L。[结论]优选出了农杆菌转化的最宜条件,遗传转化率有较大提高。[ Objective ] To establish the genetic transformation system, so as to lay foundation for the genetic transformation of tumorous stem mus- tard and other Brassica plants. [ Method ] The effects of different time of preculture, coculture, bacteria liquid concentration and dipping time on the transformation rate of tumorous stem mustard were studied with orthogonal design. [ Result ] The best conditions for genetic transformation were 3 d preeuhure ,4 d coeulture ,bacteria liquid concentration （OD600 ） O. 5 and 10 rain dipping,then the Kan concentration was 30 mg/L[ Conclution] The optimum conditions for the transformation of tumorous stem mustard were selected,which greatly improved the transformation rate.

关 键 词：茎瘤芥 正交法 遗传转化 

分 类 号：S188[农业科学—农业基础科学]