产耐热性纤维素酶菌株的分离·鉴定及其酶学性质研究  

Isolation and Identification of a Strain Producing Thermostable Cellulase and Its Enzymatic Characteristics

作　　者：杨丽娜[1] 杨明明[1] 龚月生[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《安徽农业科学》2012年第14期8103-8105,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30871813)

摘　　要：[目的]筛选耐热性纤维素降解细菌菌株,进行鉴定并研究其酶学特性。[方法]采用刚果红法从腐烂秸秆与腐殖质土壤样品中分离纤维素降解菌,通过形态学观察与16S rRNA序列分析鉴定其种属,并对该纤维素酶进行酶学性质研究。[结果]筛选到1株纤维素酶活性高的菌株NP29,经鉴定该菌株为芽孢杆菌属(Bacillus)微生物。对该菌所产纤维素酶酶学特性的研究表明,该酶反应的最适pH为4.5,最佳反应温度为65℃,具有良好的pH稳定性和热稳定性。在37℃、pH7.0的条件下,该菌株在发酵36 h后纤维素酶活性可达1.8 U/ml,且产酶量与细菌生长密切相关。[结论][ Objective ] The aim was to isolate and identify a thermostable cellulose degrading strain and study its enzymatic characteristics. [ Method ] The cellulose degrading strain was isolated from rotten straw and humus by Congo red method, and then it was identified by its morpho- logical properties and 16S rRNA sequence analysis. And its enzymatic characteristics were studied. [ Result ] A bacteria strain NP29 producing thermostable cellulase was isolated and was identified as a Bacillus. Studies on enzymatic characteristics of this strain showed that the optimum pH value was 4.5 and the optimum reaction temperature was 65 ℃ ,this cellulase had a higher pH stability and thermal stabihty. The highest cellulase activity of strain NP29 was 1.8 U/ml when growing at 37 ℃ and pH 7.0 for 36 h, the generation of this cellulase was closely related to its growth rate. [Conclusion] The study provides references for the use of cellulase resources.

关 键 词：纤维素酶 热稳定性 枯草芽孢杆菌 酶学特性 

分 类 号：S188[农业科学—农业基础科学]