复合营养素对急性束缚冷水浸泡应激诱发的Th1/Th2细胞因子变化的影响  

Effect of compound nutrients on acute immobilization and cold water-immersion stress-induced changes of Th1/Th2 cytokines

作　　者：刘亚莉[1] 毕辉[1] 樊荣[1] 李雨衡[2] 王跃民[1] 陈耀明[3] 陈景元[3] 迟素敏[1] 裴建明[1] 

机构地区：[1]第四军医大学基础医学院生理学教研室,陕西西安710032 [2]第四军医大学口腔医学系十四队,陕西西安710032 [3]第四军医大学军事预防医学系劳动与环境卫生学教研室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2012年第6期601-603,606,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(30971060;30971078)

摘　　要：目的:探讨复合营养素对大鼠急性束缚冷水浸泡应激时血清Th细胞亚群相关细胞因子变化引起的Th1/Th2失衡的影响。方法:SD雄性大鼠随机分为:正常对照组、急性应激对照组、急性应激喂复合营养素组。采用束缚冷水浸泡应激模型,急性应激30 min,应激后0 min、30 min、60 min和120 min处死动物取血,分离血清保存于-70℃备用,ELISA试剂盒检测大鼠血清中的IL-2、IL-6和TNF-α的变化。结果:急性束缚冷水浸泡应激后可引起细胞因子IL-2水平的降低,IL-6和TNF-α升高,不同时间点(0、30、60、120 min)中30min的变化最明显,应用复合营养素可削弱急性束缚冷水浸泡应激引起的IL-2水平的降低和IL-6、TNF-α的升高,同样以应激后30 min复合营养素的改善作用最显著。结论:复合营养素可以显著改善急性束缚冷水浸泡应激应激大鼠Th1/Th2细胞因子IL-2、IL-6和TNF-α的变化,提示复合营养素可改善应激大鼠的免疫调节功能,恢复Th1/Th2平衡,对于提高机体抗应激能力、减轻应激所造成的损伤可能具有一定的作用。AIM： To investigate the effect of compound nutrients on Thl/Th2 imbalance caused by changes in cytokines of Th cell subsets, interleukjn （IL）-2, IL-6 and TNF-γ, in rats with acute immobilization and cold water- immersion stress. METHODS： Male SD rats were randomly assigned to three groups including normal control group （C）, acute stress group （S） and acute stress ＋ compound nutrients group （S ＋ CN）. Stress procedure was the acute immobiliza- tion and cold water-immersion. The stress rats were fed wa- ter （Group S） or compound nutrient liquid （Group S ＋CN） by a feeding needle 1 week before acute stress, and then restrained and immersed in cold water for 30 min. The control rats were given water in the same way without stress stimulation. The rats were killed and blood samples were collected 0, 30, 60 and 120 min after stress, respectively. Serum was separated by centrifugation and stored at -70（3 until assayed. The serum levels of IL-2, IL-6 and TNF-α were analyzed by an enzyme-linked immunosorbent assay （ELISA） kit. RESULTS： Acute immobilization and cold wa- ter-immersion stress reduced IL-2 level, and increased IL-6 and TNF-cc level at different time points （0, 30, 60 and 120 min） after stress, which was most obvious at 30 min. Oral administration （gavage） of compound nutrients was found to moderate the acute immobilization and cold water-immer- sion stress-induced changes in serum IL-2, IL-6 and TNF-α, which was also most significant at 30 min after stress. （CON- CLUSION= Complex nutrients can significantly alleviate the changes of Th1/Th2 cytokines in stress rats, including IL-2, IL-6 and TNF-α, which suggests that compound nutrients can improve the immune regulation function of stress rats and restore Th1Th2 balance. Compound nutrients might enhance the bodys anti-stress ability and lighten the stress- related damage, thus being a possible candidate for the therapeutic modulation of stress.

关 键 词：复合营养素 TH细胞 细胞因子 急性束缚冷水浸泡应激 

分 类 号：R151[医药卫生—营养与食品卫生学]