四甲基偶氮唑蓝法考察青蒿素和双氢青蒿素对人肝癌细胞增殖的影响  

Effects of artemisinin and dihydroartemisinin on proliferation of human hepatocellular carcinoma cells via measurement of trititaed thymidine incorporation

作　　者：李赟[1] 王燕[1] 关志男[1] 张淑秋[1] 

机构地区：[1]山西医科大学药学院,太原030001

出　　处：《中国药物与临床》2012年第5期552-554,共3页Chinese Remedies & Clinics

基　　金：国家自然科学基金(30973589);山西省青年科技研究基金(2009021040-1);山西省留学人员科研资助项目(2009-47)

摘　　要：目的考察不同浓度和不同时间青蒿素和双氢青蒿素对人肝癌细胞(HepG2细胞)增殖的影响。方法取对数生长期的HepG2细胞为观察对象,分别给予不同浓度青蒿素(2、5、10、30、50、70、100μmol/L)和双氢青蒿素(1、3、5、10、15、20、25μmol/L)处理,于给药后24h、48h、72h采用四甲基偶氮唑蓝(MTT)法考察药物的细胞毒作用。结果 青蒿素和双氢青蒿素作用48h后,细胞的半数抑制浓度IC50分别为68,16μmol/L。与空白对照组相比,各药物组均不同程度的抑制了正常HepG2细胞的增殖,并表现出时间依赖性和剂量依赖性。结论青蒿素和双氢青蒿素在体外对HepG2细胞的增殖有明显的抑制作用,并随浓度的增大细胞毒作用也随之增大。Objective To explore the effects of artemisinin （ART） and dihydroartemisinin （DHA） with various concentrations and at different time points on proliferation of human hepatocellular carcinoma cells （HepG2）. Methods HepG2 cells in logarithmic growth phase were treated with ART （2, 5, 10, 30, 50, 70 and 100 μmol/L） and DHA （1, 3, 5, 10, 15, 20 and 25 μmol/L） and were subjected to cytotoxicity assessment using measurement of trititaed thymidine incorporation （MTT） at hours 24, 48 and 72, respectively. Results The half inhibition concentration （IC 50 ） of ART and DHA was 68 μmol/L and 16 μmol/L at hour 48, respectively. Compared with control group, both ART and DHA of various concentrations inhibited HepG2 proliferation in a doseand time-dependent fashion. Conclusion Both ART and DHA show evidence of significant inhibition of HepG2 cell proliferation in vitro in a dose-dependent manner.

关 键 词：青蒿素 双氢青蒿素 人肝癌细胞 

分 类 号：R735.7[医药卫生—肿瘤]