阿尔茨海默病大鼠海马胆固醇水平升高对tau蛋白磷酸化程度增加的影响  

Effect of high cholesterol levels in the hippocampus on tau protein phosphorylation increasing

作　　者：覃红斌[1] 魏蕾[2] 张京伟[3] 唐军民[4] 

机构地区：[1]湖北民族学院医学院,湖北恩施445000 [2]武汉大学基础医学院 [3]武汉大学中南医院 [4]北京大学基础医学院组织学胚胎学教研室

出　　处：《中国老年学杂志》2012年第11期2312-2314,共3页Chinese Journal of Gerontology

基　　金：湖北省自然科学基金〔2008CDB222〕

摘　　要：目的观察海马胆固醇含量升高后tau蛋白磷酸化程度的变化,探讨其在阿尔茨海默病(AD)中可能的作用。方法水迷宫训练大鼠7 d筛选合格大鼠后,海马直接注射不同剂量胆固醇,72 h后水迷宫检测行为学变化,并以免疫印迹和免疫组织化学方法检测大鼠海马tau蛋白磷酸化水平。结果注射胆固醇后72 h,中高剂量组大鼠水迷宫潜伏期明显延长(P<0.01),tau蛋白PHF-1位点磷酸化水平明显增高(P<0.01),tau-1明显降低(P<0.01),总tau蛋白(R134d)无明显变化。结论海马胆固醇水平升高可导致tau蛋白磷酸化程度增加,可能参与AD的形成。Objective To observe the phosphorylation of tau protein after the step up of cholesterol in hippocampus and investigate the possible function of cholesterol in Alzheimer＇s disease （AD）. Methods Rats were sieved with morris water maze for 7 days, injected with different dosage of cholesterol into hippocampus. Then ethology, abnormal tau phosphorylation and cholesterol level were examined by morris water maze, Western blot and immunohistochemistry. Results After injected 72 h with different dosage of cholesterol into hippocam- pus, the water maze latency of middle and high dose group were obvious extended （P 〈 0. 01 ）, the phospborylation level of PHF-1 were increased （ P 〈 0. 01 ） , tau-1 were decreased （ P 〈 0.01 ） , total tau （R134d） were no change. Conclusions The raise of cholesterol in brain which inducing the step up of tau phosphorylation could be a possible way to form AD.

关 键 词：AD 胆固醇水平 tan蛋白磷酸化 神经退行性变 

分 类 号：R749[医药卫生—神经病学与精神病学]