高效液相色谱-电喷雾/质谱法测定青稞幼苗提取物氨基酸  

Determination of Amino Acids from Highland Barley Seedlings Extract by HPLC-ESI/MS

作　　者：王萍[1,2] 陈涛[1,2] 杜玉枝[1] 沈裕虎[1] 李玉林[1] 

机构地区：[1]中国科学院西北高原生物研究所,西宁市新宁路23号810008 [2]中国科学院研究生院

出　　处：《光谱实验室》2012年第3期1351-1355,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-EW-J-26)

摘　　要：以2-[2-(7H-二苯并[a,g]咔唑-乙氧基)-乙基]氯甲酸酯(DBCEC-Cl)作为柱前衍生试剂,采用高效液相色谱-电喷雾/质谱法(HPLC-ESI/MS)对青稞幼苗提取物中的氨基酸进行了测定。结果表明,青稞幼苗提取物中含有13种氨基酸,总含量为81.6mg/g,其中有人体自身不能合成的6种重要氨基酸[赖氨酸(Lys)、蛋氨酸(Met)、亮氨酸(Leu)、异亮氨酸(Ile)、苏氨酸(Thr)和缬氨酸(Val)],含量达41.8mg/g,占总氨基酸的51.2%。该方法灵敏、可靠、重现性好,是一种理想的全谱氨基酸分析方法。The amino acids in highland barley seedlings extract was determined by HPLC-ESI/MS with 2-[2-（dibenzocarbazole）-ethoxy] ethyl chloroformate（DBCEC-Cl） as the sensitive pre-column derivatization reagent.The results showed that there were 13 kind of amino acids in highland barley seedlings extract,and the total content was 81.6mg/g,and there were 6 kinds of amino acids that couldn＇t be synthesized by the body itself,including lysine（Lys）,methionine（Met）,leucine（Leu）,isolencine（Ile）,threonine（Thr） and valine（Val）,and their contents amounted to 41.8mg/g with accounting for 51.2% of the total amino acids.The method is sensitive and reliable with good repeatability,that is as the ideal analysis of full-spectrum amino acids in highland barely seedlings.

关 键 词：青稞 氨基酸 高效液相色谱-电喷雾/质谱法 2-[2-(7H-二苯并[a g]咔唑-乙氧基)-乙基]氯甲酸酯 

分 类 号：O657.63[理学—分析化学]