Folin-Ciocalteu比色法测定石榴皮提取物中的多酚  

Determination of Polyphenols in Pomegranate Peel Extracts by Folin-Ciocalteu Colorimetry

作　　者：朱彩平[1] 夏德水[1] 

机构地区：[1]陕西师范大学食品工程与营养科学学院,西安市长安南路199号710062

出　　处：《光谱实验室》2012年第3期1356-1360,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：中央高校基本科研业务费专项资金(GK200902044)

摘　　要：研究Folin-Ciocalteu比色法测定石榴皮提取物中多酚含量的优化条件,并对该方法的稳定性、精密度、重现性、回收率进行了实验。结果表明,石榴皮提取物中多酚含量测定的最佳条件为:Folin-Ciocalteu试剂1.00mL、10.0g/100mL Na2CO33.00mL、反应温度25℃、反应时间120min、测定波长672nm,所用没食子酸标准品在10—100μg/mL范围内与吸光度呈良好的线性关系(y=0.0098x+0.0148,R2=0.9982)。该法简便、快速、准确,是检测石榴皮提取物中总多酚的可靠方法。The optimal conditions of the determination of the contents of polyphenols in pomegranate peel extracts by Folin-Ciocalteu colorimetry were investigated,and the stability,precision,repeatability and recovery of the method were tested.The optimal conditions of the determination of polyphenols in pomegranate peel extracts were obtained as follows：Folin-Ciocalteu reagent of 1.00mL,10.0g/100mL Na2CO3 of 3.00mL,the reaction temperature of 25℃,reaction time of 120min and detection wavelength of 672nm.There was a good linear relation ship between the concentration of gallic acid and absorbance in the range of 10—100μg/mL（y=0.0098x＋0.0148,R2=0.9982）.The method is simple,rapid and accurate,that is the reliable method for detection of total polyphenols in pomegranate peel extracts.

关 键 词：石榴皮 多酚 分光光度法 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.32[轻工技术与工程—食品科学与工程]