基于AFM的药物刺激前后淋巴瘤活细胞的形貌及弹性的变化(英文)  

Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy

作　　者：李密[1,2] 刘连庆[1] 席宁[1,3] 王越超[1] 董再励[1] 肖秀斌[4] 张伟京[4] 

机构地区：[1]中国科学院沈阳自动化研究所,机器人学国家重点实验室,沈阳110016 [2]中国科学院研究生院,北京100049 [3]密歇根州立大学电子与计算机工程系,美国东兰辛48824 [4]军事医学科学院附属医院淋巴科,北京100071

出　　处：《物理化学学报》2012年第6期1502-1508,共7页Acta Physico-Chimica Sinica

基　　金：The project was supported by the National Natural Science Foundation of China(60904095,61175103);National High Technology Research and Development Program of China(863)(2009AA03Z316);Chinese Academy of Sciences Foreign Experts Affairs International Partnership Program for Creative Research Teams~~

摘　　要：原子力显微镜(AFM)的发明为研究单个活细胞的形貌结构及物理特性提供了新的技术手段.然而,由于缺少合适的固定方法,利用AFM对动物悬浮活细胞的形貌进行高分辨率成像还面临着巨大的挑战.本文提出一种基于微柱阵列和静电吸附相结合的动物悬浮细胞固定方法.通过微柱阵列的机械钳制和多聚赖氨酸的静电吸附实现了对单个淋巴瘤B细胞的固定,并在此基础上利用AFM动态观测了不同浓度Rituximab刺激下淋巴瘤B细胞的表面形貌及弹性的变化.经过0.2 mg·mL-1的Rituximab刺激2 h后,细胞表面的褶皱增加,细胞的杨氏模量从196 kPa减小到183 kPa.经过0.5 mg·mL-1的Rituximab刺激2 h后,细胞形貌发生显著变化并出现突起结构,细胞的杨氏模量从234 kPa减小到175 kPa.实验结果表明淋巴瘤细胞形貌和弹性变化的幅度随着Rituximab刺激浓度的增加而增加,加深了对Rituximab作用效果的认识.Atomic force microscopy（AFM） provides a means for characterizing the surface topography and biophysical properties of individual living cells under near-physiological conditions.However,owing to the lack of adequate cellular immobilization methods,AFM imaging of living,suspended mammalian cells is still a big challenge.In this paper,a method is presented for immobilizing individual living B lymphoma cells that combines mechanical trapping with pillar arrays and electrostatic adsorption with poly-L-lysine.In this way,the topography and elasticity changes of individual B lymphoma cells that were stimulated with different concentrations of Rituximab were observed and measured dynamically.When the cell is stimulated by 0.2 mg·mL-1Rituximab for 2 h,the cell topography becomes more corrugated and Young smodulus decreases from 196 to 183 kPa.When the cell is stimulated by 0.5 mg·mL-1Rituximab for 2 h,thecell topography changes more significantly and some tubercles appear,and Young s modulus decreasesfrom 234 to 175 kPa.These results thus provide a unique insight into the effects of Rituximab on individualcells.

关 键 词：原子力显微镜 淋巴瘤 弹性 力曲线 杨氏模量 

分 类 号：R733.1[医药卫生—肿瘤]