白及须根和块茎总酚含量的测定  

Determination of Total Polyphenol Content in Different Parts of Rhizoma Bletillae Striatae

作　　者：周云凯[1] 李伟平[1] 田莎莎[1] 马丹丹[1] 蒋福升[1] 丁志山[1] 

机构地区：[1]浙江中医药大学,杭州310053

出　　处：《中国实验方剂学杂志》2012年第10期161-164,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：浙江省公益性技术应用研究计划项目(2011C22047);江山市面上科技计划项目(2010B16);浙江省大学生科技创新活动计划项目(2010R410058)

摘　　要：目的:测定白及块茎和须根中总酚的含量。方法:以没食子酸为对照品,采用folin-酚比色法测定白及块茎和须根中的总酚含量。采用TLC对白及须根和块茎进行定性比较。结果:采用folin-酚比色法测定白及中总酚含量,准确度好,精密度高。没食子酸对照品在5～25μg的范围内线性良好,标准曲线方程为Y=0.033 2X+0.000 1。白及须根中的总酚含量为0.895%,块茎中的总酚含量为0.394%。TLC结果表明,白及须根的斑点比块茎多。结论:白及须根中总酚含量高于块茎。Objective： To determine the total polyphenol content in the fibrous roots and tubers of Rhizoma Bletillae Striatae. Method： The content of total polyphenol in the fibrous roots and tubers of Rhizoma Bletillae Striatae was detected by folin colorimetry method using gallic acid as index, then using TLC method to qualitative compare fibrous roots with tubers of Rhizoma Bletillae Striatae. Result： There was a good linearity in the range of 5-25 μg for the gallic acid, the standard curve equation was Y = 0. 033 2X ＋ 0.000 1. The total polyphenol content in the fibrous roots was 0. 895% while tubers was 0. 394%. Conclusion： the total Polyphenol content in the fibrous roots of Rhizoma Bletillae Striatae is higher then tuber.

关 键 词：白及 总酚 须根 块茎 薄层色谱 

分 类 号：R284.1[医药卫生—中药学]