百合总皂苷提取工艺及抗抑郁活性研究  

Extraction and Purification Techniques and Antidepression Activity of Total Saponins from Bulbs of Lilium brownii

作　　者：傅春燕[1] 刘永辉 李明娟[1] 王晓慧[1] 尹文清[3] 

机构地区：[1]邵阳医学高等专科学校 [2]湖南省新邵县红十字会医院,邵阳422000 [3]广西师范大学化学化工学院药用资源化学与药物分子工程教育部重点实验室,桂林541004

出　　处：《天然产物研究与开发》2012年第5期682-686,共5页Natural Product Research and Development

基　　金：湖南省教育厅科研基金项目(10C0294)

摘　　要：本文研究百合总皂苷最佳提取工艺,继而采用AB-8大孔吸附树脂富集纯化百合总皂苷,并测试其抗抑郁活性。采用正交实验,考察乙醇浓度、提取次数、固液比及提取时间四个因素对该工艺的影响;采用小鼠悬尾实验和小鼠强迫游泳实验评价百合总皂苷的抗抑郁活性。确立了百合总皂苷的最佳提取工艺为:提取时间3h,乙醇浓度80%,固液比1∶10,提取次数为3次。在最佳提取工艺条件下,总皂苷提取率为1.24%。经过AB-8大孔树脂富集纯化总皂苷的含量达到62%以上。药理实验表明,百合总皂苷中剂量、小剂量能明显缩短小鼠悬尾的不动时间和游泳时间(P<0.05或者P<0.01),百合富集纯化的总皂苷部位具有较好的抗抑郁活性。Extraction and purification techniques of total saponins from bulbs of Lilium brownii F. E. Brown var. viridu- lum Baker. and the antidepressant activity of the total saponins were studied in this paper. Based on orthogonal design, four factors in extraction of saponins from bulbs of Lilium brownie were optimized： concentration of ethanol, reflux time, solid/liquid ratio and extraction times. The optimized process for extracting total saponins from bulbs of Lilium brownii was confirmed as follows：reflux time,3 h; alcohol,80% ;solid/liquid, 1 ： 10;extraction 3 times. The highest extraction rate reaches 1.24%. Then,the extract of total saponins was separated and purified by AB-8 porous resin absorption and the total saponins content reaching 62%. In antidpression effect filtration study, the mouse tail suspended experiment, mouse forced swimming experiment were performed, the result showed that the total saponins was the main antidepression component. The above results showed that the total saponins had certain antidepressant activity.

关 键 词：百合总皂苷 提取工艺 抗抑郁活性 

分 类 号：R914[医药卫生—药物化学]