高产碱性β-葡萄糖苷酶的产纤维素酶菌株的筛选  

Screening of Cellulose-producing Bacterium with High Yielding of β-Glucosidase

作　　者：苏迪[1] 徐小蓉[1] 唐婧[1] 

机构地区：[1]贵州师范大学生命科学学院,贵州贵阳550001

出　　处：《贵州农业科学》2012年第2期87-89,94,共4页Guizhou Agricultural Sciences

基　　金：贵州省自然科学基金项目[黔科合J字(2011)2180]

摘　　要：为获得高产碱性β-葡萄糖苷酶的产纤维素酶菌株,利用碱性羧甲基纤维素钠平板筛选和β-葡萄糖苷酶平板筛选法相结合,从植物腐败堆积土壤中分离到1株高产碱性β-葡萄糖苷酶的产纤维素酶克雷伯氏杆菌,并对其进行初步酶学鉴定。结果表明:该菌胞外分泌液具有内切葡聚糖酶、外切葡聚糖酶及β-葡萄糖苷酶的活性,其中以β-葡萄糖苷酶活性最高,为48.9U/mL。其所产纤维素酶的最适反应pH值为10.0,最适反应温度为35℃,且Ca2+、Mg2+、Zn2+和Mn2+等金属离子对该酶具有较强的抑制作用。In order to get a cellulase-high-yielding bacterium,CMCNa plate and β-glucosidase plate screening method were used to screen a bacteria strain,and its enzymology properties were preliminarily identified.The results showed that a facultative anaerobic strain which produced cellulase was obtained from the CMCNa plate and β-glucosidase plate screening.This strain was identified as Klebsiella sp.,and termed as strain CBG-08.The secretion of this strain showed activities of endoglucanase,cellubiohydrolase and β-glucosidase,β-glucosidase had the highest activity.The enzyme had the highest activity when pH was 10.0 and temperature was 35℃,and metal elements of Ca2＋,Mg2＋,Zn2＋ and Mn2＋ could inhibit enzyme activity.

关 键 词：纤维素酶产生菌 Β-葡萄糖苷酶 筛选 碱性 酶学性质 

分 类 号：S182[农业科学—农业基础科学]