野生二粒小麦高分子量谷蛋白亚基组成的多态性研究  

Diversity Study on the Subunit Composition of High Molecular Weight Glutenin in Wild Emmer

作　　者：张大乐[1] 李黎[1] 方圆[1] 苏亚蕊[1] 李玉阁[1] 李锁平[1] 

机构地区：[1]河南大学生命科学学院,河南开封475004

出　　处：《河南大学学报（自然科学版）》2012年第3期286-290,共5页Journal of Henan University:Natural Science

基　　金：国家自然科学基金项目(30971774);"十二五"农村领域国家科技计划课题(2011BAD07B01-1);河南省教育厅自然科学研究项目(2010B210002)

摘　　要：对来自以色列的38份野生二粒小麦的高分子量谷蛋白亚基(high molecular weight glutenin subunits,HMW-GS)组成进行SDS-PAGE检测,发现其HMW-GS具有较高的遗传多样性.在Glu-A1和Glu-B1位点共有18种可确定的亚基变异类型.其中,A1位点上的亚基1、2*(出现频率分别为60.53%和13.16%)和B1位点上的亚基13+16、14+15与17+18为优质亚基(出现频率分别为26.32%、7.89%和2.63%).同时出现了许多普通小麦所稀有或缺乏的特异亚基类型,如6+8*,7*+9,6+9*,7+9*,20,22等,在2份材料中出现了1种未命名的新亚基类型.SDS-PAGE detection was conducted on 38 shares of wild emmer from Israel and it was found that their high molecular weight glutenin subunits （HMW-GS） had higher genetic diversity. 18 types of confirmable subunit variations appeared on the loci of Glu A1 and GIu-B1. Among them, subunits 1 and 2 （with occurrence frequencies 60. 53% and 13.16%） on A1 locus and subunits 13＋16, 14＋15 and 17＋18 （with occurrence frequencies 26.32%, 7.89% and 2.63%, respectively ） on B1 locus were high-quality subunits. In addition, many peculiar subunit types rare in common wheat were found, such as 6＋8, 7 ＋9, 6＋9, 7＋9, 20, 22, etc. And one new unnamed subunit type occurred in two shares of materials.

关 键 词：野生二粒小麦 高分子量谷蛋白亚基 SDS-PAGE 

分 类 号：S512.024[农业科学—作物学]