香菇多糖联合依地酸钙钠对铅中毒小鼠学习记忆功能及海马NO,nNOS的影响  

The Influence of Lentinan United Calcium Disodium Edetate on Learning and Memory,NO,nNOS in Hippocampus of Lead Poisoning Mice

作　　者：孙静[1] 周雅静[2] 韩璇[2] 史进忠 刘会芳[4] 

机构地区：[1]河北大学附属医院,河北保定071000 [2]河北大学基础医学院,河北保定071000 [3]保定市第一医院,河北保定071000 [4]河北大学药学院,河北省药物质量研究重点实验室,河北保定071000

出　　处：《河北师范大学学报（自然科学版）》2012年第3期297-300,306,共5页Journal of Hebei Normal University：Natural Science

基　　金：河北省科学技术研究与发展计划(10276428);河北省中医药管理局科研计划(2011035)

摘　　要：将50只小鼠随机分成空白对照组、模型对照组、阳性对照组、香菇多糖低剂量组、香菇多糖高剂量组5个组,用Morris水迷宫检测小鼠学习记忆功能,微分电位溶出法测定血铅含量,硝酸还原酶法检测NO含量,免疫组化方法观察nNOS阳性细胞表达.结果表明,香菇多糖高剂量组能显著改善铅中毒小鼠的学习记忆功能.与模型对照组相比,香菇多糖高剂量组能显著降低血铅浓度(P<0.01),提高海马NO含量(P<0.01),且增强nNOS阳性神经元表达CA1(P<0.05),CA3(P<0.01).这提示香菇多糖联合依地酸钙钠能改善铅中毒小鼠的学习记忆功能,其作用机制可能与海马NO含量增高及nNOS神经元表达增强有关.The 50 mice were randomly divided into five groups, blank group, negative control group, positive control group, lentinan low dose group and high dose group. Morris water maz was used to monitor the learning and memory ability. The lead concentrations of blood were measured by electric potential stripping method. Inthe mean time,The content of NO was tested by nitrate reductase method. The expression of nNOS positive cells were observed with immunohistochemistry. The results indicated that compared with that of negative control group, the learning and memory ability of lead poisoning mice could be markedly improved by lentinan high dose group lentinan high dose group, and the lead concentrations of blood could be significantly decreased（P 〈 0.01）. The hippocampal intracellular NO content was obviously increased （ P 〈 0.01 ）. The nNOS positive cells of hippecarnpus were increased CA1（ P 〈 0.05）, CA3 （ P 〈 0.01）. Lentinan and calcium disodium edetate could improve the learning and memory on lead poisoning mice. The mechanism may be related with increasing NO content and the expression of nNOS positive cells in hippoeampus.

关 键 词：香菇多糖 依地酸钙钠 铅中毒 学习记忆 一氧化氮 神经元型一氧化氮合酶 

分 类 号：R745[医药卫生—神经病学与精神病学]